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NFkappaB p65 elisa kit :: Rat nuclear factor-?B p65, NF-kappaB p65 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704916
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $605 +1 FREE 8GB USB
96-Strip-Wells  /  $870 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,950 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,535 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

nuclear factor-?B p65, NF-kappaB p65 (NFkappaB p65), ELISA Kit

 Also Known As   

Rat nuclear factor-?B p65, NF-kappaB p65 ELISA Kit

 Product Synonym Names    Rat nuclear factor-kappaB p65; NF-kappaB p65 ELISA Kit; nuclear factor-kappaB p65; NF-kappaB p65
 Product Gene Name   

NFkappaB p65 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    PDF Manual
 MBS704916 Testing Data    Testing Data PDF
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat NF-kB p65. No significant cross-reactivity or interference between rat NF-kB p65 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.56 pg/ml -100 pg/ml.
 Sensitivity    The minimum detectable dose of rat NF-kB p65 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of NFkappaB p65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NFkappaB p65 purchase    MBS704916 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear factor-?B p65, NF-kappaB p65 (NFkappaB p65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range of 1.56 pg/ml -100 pg/ml in biological research samples containing NFkappaB p65, with an estimated sensitivity of The minimum detectable dose of rat NF-kB p65 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704916 kit is based on NFkappaB p65 antibody-NFkappaB p65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFkappaB p65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFkappaB p65. Appropriate sample types include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NFkappaB p65 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NFkappaB p65 elisa kit    NFkappaB p65 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for NFkappaB p65. It may not necessarily be applicable to this product.
 NCBI GI #    117616548
 NCBI Accession #    ABK42292.1 [Other Products]
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 NCBI Official Full Name    NFkappaB p65
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Rat nuclear factor-?B p65, NF-kappaB p65 ELISA Kit
 Catalog #  Product Name
 MBS704916  Rat nuclear factor-?B p65, NF-kappaB p65 ELISA Kit
Products associated with NFkappaB p65 elisa kitPathways associated with NFkappaB p65 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with NFkappaB p65 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with NFkappaB p65 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NFkappaB p65 and Bone
 Connective Tissue Antibodies  >4 publications with NFkappaB p65 and Connective Tissue
 Heart Antibodies  >3 publications with NFkappaB p65 and Heart
 Muscle Antibodies  >3 publications with NFkappaB p65 and Muscle
 Brain Antibodies  >3 publications with NFkappaB p65 and Brain
 Vascular Antibodies  >2 publications with NFkappaB p65 and Vascular
 Embryonic Tissue Antibodies  >2 publications with NFkappaB p65 and Embryonic Tissue
 Ear Antibodies  >1 publications with NFkappaB p65 and Ear
 Kidney Antibodies  >1 publications with NFkappaB p65 and Kidney
 Umbilical Cord Antibodies  >1 publications with NFkappaB p65 and Umbilical Cord
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