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PAMY elisa kit :: Rat pancreatic amylase (PAMY) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS269618
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

pancreatic amylase (PAMY), ELISA Kit

★Popular Item★
 Also Known As   

Rat pancreatic amylase (PAMY) ELISA Kit

 Product Gene Name   

PAMY elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PAMY concentration
 Assay Type    Sandwich
 Detection Range    2000 U/L-31.2 U/L
 Sensitivity    12 U/L.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PAMY elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PAMY purchase    MBS269618 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the pancreatic amylase (PAMY) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 U/L-31.2 U/L in biological research samples containing PAMY, with an estimated sensitivity of 12 U/L. The ELISA analytical biochemical technique of the MBS269618 kit is based on PAMY antibody-PAMY antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAMY antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAMY. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PAMY concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PAMY elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PAMY monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PAMY elisa kit    PAMY elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS269618. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PAMY. It may not necessarily be applicable to this product.
 NCBI GI #    13928684
 NCBI GeneID    497039
 NCBI Accession #    NP_113690.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031502.1 [Other Products]
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 UniProt Related Accession #    P00689 [Other Products]
 Molecular Weight    57,177 Da
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 NCBI Official Full Name    pancreatic alpha-amylase
 NCBI Official Synonym Full Names    amylase 2a3
 NCBI Official Symbol    Amy2a3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    pancreatic alpha-amylase; PA; pancreatic amylase; amylase 2, pancreatic; 1,4-alpha-D-glucan glucanohydrolase
 UniProt Protein Name    Pancreatic alpha-amylase
 UniProt Synonym Protein Names   
1,4-alpha-D-glucan glucanohydrolase
 UniProt Gene Name    Amy2 [Similar Products]
 UniProt Synonym Gene Names    PA [Similar Products]
 UniProt Entry Name    AMYP_RAT
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 UniProt Comments for PAMY    AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Hydrolase; Secreted; EC; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose

Cellular Component: extracellular space

Molecular Function: cation binding; alpha-amylase activity; protein binding; calcium ion binding; chloride ion binding

Biological Process: carbohydrate catabolic process; carbohydrate metabolic process
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 Research Articles on PAMY    1. Comparative proteomic analysis revealed that amylase levels were decreased by twofold in pancreases from hyperlipidemic rats compared with control rats.Pancreatic amylase protein levels were also decreased in tissue from hyperlipidemic rats.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PAMY elisa kit
 Products by Pathway  Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
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