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PRG4 elisa kit :: Rat proteoglycan 4 (PRG4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705095
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
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 Product Name   

proteoglycan 4 (PRG4), ELISA Kit

★Popular Item★
 Also Known As   

Rat proteoglycan 4, PRG4 ELISA Kit

 Product Synonym Names    Rat proteoglycan 4 (PRG4) ELISA kit; GS1-174L6.2; CACP; FLJ32635; HAPO; JCAP; MSF; SZP; bG174L6.2; OTTHUMP00000033580; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor) ; lubricin; megakaryocyte stimulat; proteoglycan 4 (PRG4)
 Product Gene Name   

PRG4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705095 Testing Data    Testing Data PDF
 OMIM    604283
 3D Structure    ModBase 3D Structure for Q92954
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat PRG4. No significant cross-reactivity or interference between Rat PRG4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    1.56 ng/ml-100 ng/ml
 Sensitivity    0.39 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRG4 purchase    MBS705095 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the proteoglycan 4 (PRG4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56 ng/ml-100 ng/ml in biological research samples containing PRG4, with an estimated sensitivity of 0.39 ng/ml. The ELISA analytical biochemical technique of the MBS705095 kit is based on PRG4 antibody-PRG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRG4 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRG4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PRG4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PRG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PRG4 elisa kit    PRG4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705095. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRG4. It may not necessarily be applicable to this product.
 NCBI GI #    189181720
 NCBI GeneID    10216
 NCBI Accession #    NP_001121180.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001127708.1 [Other Products]
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 UniProt Primary Accession #    Q92954 [Other Products]
 UniProt Secondary Accession #    Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49 [Other Products]
 UniProt Related Accession #    Q92954 [Other Products]
 Molecular Weight    151,077 Da
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 NCBI Official Full Name    proteoglycan 4 isoform B
 NCBI Official Synonym Full Names    proteoglycan 4
 NCBI Official Symbol    PRG4 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    proteoglycan 4; lubricin; superficial zone proteoglycan; megakaryocyte stimulating factor; megakaryocyte-stimulating factor; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor )
 UniProt Protein Name    Proteoglycan 4
 UniProt Synonym Protein Names   
Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part
 Protein Family    Proteoglycan
 UniProt Gene Name    PRG4 [Similar Products]
 UniProt Synonym Gene Names    MSF; SZP [Similar Products]
 UniProt Entry Name    PRG4_HUMAN
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 NCBI Summary for PRG4    The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRG4    Function: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.2 PublicationsManual assertion based on experiment in:Ref.6
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 Research Articles on PRG4    1. Lubricin is transcribed, translated, and expressed by ocular surface epithelia. Lubricin presence significantly reduces friction between the cornea and conjunctiva.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PRG4 elisa kitOrgans/Tissues associated with PRG4 elisa kit
 Disease Name  Pubmed Publications
 Pericarditis Antibodies  >12 publications with PRG4 and Pericarditis
 Necrosis Antibodies  >6 publications with PRG4 and Necrosis
 Inflammation Antibodies  >4 publications with PRG4 and Inflammation
 Jacobs syndrome Antibodies  >3 publications with PRG4 and Jacobs syndrome
 Disease Models, Animal Antibodies  >3 publications with PRG4 and Disease Models, Animal
 Contracture Antibodies  >3 publications with PRG4 and Contracture
 Hyperplasia Antibodies  >3 publications with PRG4 and Hyperplasia
 Hypertrophy Antibodies  >2 publications with PRG4 and Hypertrophy
 Lung Neoplasms Antibodies  >1 publications with PRG4 and Lung Neoplasms
 Disorders of Sex Development Antibodies  >1 publications with PRG4 and Disorders of Sex Development
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >10 publications with PRG4 and Connective Tissue
 Vascular Antibodies  >4 publications with PRG4 and Vascular
 Liver Antibodies  >4 publications with PRG4 and Liver
 Embryonic Tissue Antibodies  >3 publications with PRG4 and Embryonic Tissue
 Lung Antibodies  >2 publications with PRG4 and Lung
 Heart Antibodies  >2 publications with PRG4 and Heart
 Adipose Tissue Antibodies  >2 publications with PRG4 and Adipose Tissue
 Thymus Antibodies  >1 publications with PRG4 and Thymus
 Eye Antibodies  >1 publications with PRG4 and Eye
 Uncharacterized Tissue Antibodies  >1 publications with PRG4 and Uncharacterized Tissue
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