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SIRT1 elisa kit :: Rat sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS706792
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (SIRT1), ELISA Kit

★Popular Item★
 Also Known As   

Rat NAD-dependent protein deacetylase sirtuin-1, SIRT1 ELISA Kit

 Product Synonym Names    Rat NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit; RP11-57G10.3; SIR2L1; SIR2alpha; sir2-like 1; sirtuin 1; sirtuin type 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
 Product Gene Name   

SIRT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706792 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q923E4
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat SIRT1. No significant cross-reactivity or interference between rat SIRT1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    34.3 pg/ml-2200 pg/ml.
 Sensitivity    The minimum detectable dose of rat SIRT1 is typically less than 8.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SIRT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SIRT1 purchase    MBS706792 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (SIRT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 34.3 pg/ml-2200 pg/ml in biological research samples containing SIRT1, with an estimated sensitivity of The minimum detectable dose of rat SIRT1 is typically less than 8.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS706792 kit is based on SIRT1 antibody-SIRT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIRT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIRT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SIRT1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SIRT1 elisa kit    SIRT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706792. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SIRT1. It may not necessarily be applicable to this product.
 NCBI GI #    9790229
 NCBI GeneID    93759
 NCBI Accession #    NP_062786.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_019812.2 [Other Products]
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 UniProt Primary Accession #    Q923E4 [Other Products]
 UniProt Secondary Accession #    Q9QXG8 [Other Products]
 UniProt Related Accession #    Q923E4 [Other Products]
 Molecular Weight    80,372 Da
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 NCBI Official Full Name    NAD-dependent protein deacetylase sirtuin-1 isoform 1
 NCBI Official Synonym Full Names    sirtuin 1
 NCBI Official Symbol    Sirt1 [Similar Products]
 NCBI Official Synonym Symbols   
Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
[Similar Products]
 NCBI Protein Information    NAD-dependent protein deacetylase sirtuin-1; mSIR2a; sir2-like 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1
 UniProt Protein Name    NAD-dependent protein deacetylase sirtuin-1
 UniProt Synonym Protein Names   
Regulatory protein SIR2 homolog 1; SIR2-like protein 1; SIR2alpha; Sir2; mSIR2a
 Protein Family    NAD-dependent protein deacetylase sirtuin
 UniProt Gene Name    Sirt1 [Similar Products]
 UniProt Synonym Gene Names    Sir2l1; Sir2; mSIR2a; 75SirT1 [Similar Products]
 UniProt Entry Name    SIR1_MOUSE
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 UniProt Comments for SIRT1    Function: NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD+/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. Deacetylates H2A and 'Lys-26' of HIST1H1E. Deacetylates 'Lys-16' of histone H4 (in vitro). Involved in NR0B2/SHP corepression function through chromatin remodeling: Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Proposed to contribute to genomic integrity via positive regulation of telomere length; however, reports on localization to pericentromeric heterochromatin are conflicting. Proposed to play a role in constitutive heterochromatin (CH) formation and/or maintenance through regulation of the available pool of nuclear SUV39H1. Upon oxidative/metabolic stress decreases SUV39H1 degradation by inhibiting SUV39H1 polyubiquitination by MDM2. This increase in SUV39H1 levels enhances SUV39H1 turnover in CH, which in turn seems to accelerate renewal of the heterochromatin which correlates with greater genomic integrity during stress response. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce transcription-dependent proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Deacetylates MYC, promotes the association of MYC with MAX and decreases MYC stability leading to compromised transformational capability. Deacetylates FOXO3 in response to oxidative stress thereby increasing its ability to induce cell cycle arrest and resistance to oxidative stress but inhibiting FOXO3-mediated induction of apoptosis transcriptional activity; also leading to FOXO3 ubiquitination and protesomal degradation. Appears to have a similar effect on MLLT7/FOXO4 in regulation of transcriptional activity and apoptosis. Deacetylates DNMT1; thereby impairs DNMT1 methyltransferase-independent transcription repressor activity, modulates DNMT1 cell cycle regulatory function and DNMT1-mediated gene silencing. Deacetylates RELA/NF-kappa-B p65 thereby inhibiting its transactivating potential and augments apoptosis in response to TNF-alpha. Deacetylates HIF1A, KAT5/TIP60, RB1 and HIC1. Deacetylates FOXO1, which increases its DNA binding ability and enhances its transcriptional activity leading to increased gluconeogenesis in liver. Inhibits E2F1 transcriptional activity and apoptotic function, possibly by deacetylation. Involved in HES1- and HEY2-mediated transcriptional repression. In cooperation with MYCN seems to be involved in transcriptional repression of DUSP6/MAPK3 leading to MYCN stabilization by phosphorylation at 'Ser-62'. Deacetylates MEF2D. Required for antagonist-mediated transcription suppression of AR-dependent genes which may be linked to local deacetylation of histone H3. Represses HNF1A-mediated transcription. Required for the repression of ESRRG by CREBZF. Modulates AP-1 transcription factor activity. Deacetylates NR1H3 AND NR1H2 and deacetylation of NR1H3 at 'Lys-434' positively regulates transcription of NR1H3:RXR target genes, promotes NR1H3 proteosomal degradation and results in cholesterol efflux; a promoter clearing mechanism after reach round of transcription is proposed. Involved in lipid metabolism. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG which probably involves association with NCOR1 and SMRT/NCOR2. Deacetylates ACSS2 leading to its activation, and HMGCS1. Involved in liver and muscle metabolism. Through deacteylation and activation of PPARGC1A is required to activate fatty acid oxidation in skeletel muscle under low-glucose conditions and is involved in glucose homeostasis. Involved in regulation of PPARA and fatty acid beta-oxidation in liver. Involved in positive regulation of insulin secretion in pancreatic beta cells in response to glucose; the function seems to imply transcriptional repression of UCP2. Proposed to deacetylate IRS2 thereby facilitating its insulin-induced tyrosine phosphorylation. Deacetylates SREBF1 isoform SREBP-1C thereby decreasing its stability and transactivation in lipogenic gene expression. Involved in DNA damage response by repressing genes which are involved in DNA repair, such as XPC and TP73, deacetylating XRCC6/Ku70, and faciliting recruitment of additional factors to sites of damaged DNA, such as SIRT1-deacetylated NBN can recruit ATM to initiate DNA repair and SIRT1-deacetylated XPA interacts with RPA2. Also involved in DNA repair of DNA double-strand breaks by homologous recombination and specifically single-strand annealing independently of XRCC6/Ku70 and NBN. Transcriptional suppression of XPC probably involves an E2F4:RBL2 suppressor complex and protein kinase B (AKT) signaling. Transcriptional suppression of TP73 probably involves E2F4 and PCAF. Deacetylates WRN thereby regulating its helicase and exonuclease activities and regulates WRN nuclear translocation in response to DNA damage. Deacetylates APEX1 at 'Lys-6' and 'Lys-7' and stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1. Increases p53/TP53-mediated transcription-independent apoptosis by blocking nuclear translocation of cytoplasmic p53/TP53 and probably redirecting it to mitochondria. Deacetylates XRCC6/Ku70 at 'Lys-537' and 'Lys-540' causing it to sequester BAX away from mitochondria thereby inhibiting stress-induced apoptosis. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and MAP1LC3B/ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Proposed to play role in regulation of STK11/LBK1-dependent AMPK signaling pathways implicated in cellular senescence which seems to involve the regulation of the acetylation status of STK11/LBK1. Can deacetylate STK11/LBK1 and thereby increase its activity, cytoplasmic localization and association with STRAD; however, the relevance of such activity in normal cells is unclear. In endothelial cells is shown to inhibit STK11/LBK1 activity and to promote its degradation. Deacetylates SMAD7 at 'Lys-64' and 'Lys-70' thereby promoting its degradation. Deacetylates CIITA and augments its MHC class II transactivation and contributes to its stability. Deacteylates MECOM/EVI1. Isoform 2 is shown to deacetylate 'Lys-382' of p53/TP53, however with lower activity than isoform 1. In combination, the two isoforms exert an additive effect. Isoform 2 regulates p53/TP53 expression and cellular stress response and is in turn repressed by p53/TP53 presenting a SIRT1 isoform-dependent auto-regulatory loop. Deacetylates PML at 'Lys-487' and this deacetylation promotes PML control of PER2 nuclear localization. During the neurogenic transition, repress selective NOTCH1-target genes through histone deacetylation in a BCL6-dependent manner and leading to neuronal differentiation.30 PublicationsManual assertion based on experiment in:Ref.3

Catalytic activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

Cofactor: Binds 1 zinc ion per subunit

Enzyme regulation: Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone). MAPK8/JNK1 and RPS19BP1/AROS act as positive regulators of deacetylation activity Inhibited by nicotinamide. Negatively regulated by CCAR2 1 PublicationManual assertion based on experiment in:Ref.3
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 Research Articles on SIRT1    1. when SirT1siRNA or AMPKa1-siRNA was transfected into the 3T3-L1 adipocytes prior to treatment with insulin and EPS, there was a significant increase in IL6 and TNFa mRNA abundance
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SIRT1 elisa kitPathways associated with SIRT1 elisa kit
 Reference Product  PubMed Publications
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Diseases associated with SIRT1 elisa kitOrgans/Tissues associated with SIRT1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >318 publications with SIRT1 and Inflammation
 Cardiovascular Diseases Antibodies  >291 publications with SIRT1 and Cardiovascular Diseases
 Insulin Resistance Antibodies  >206 publications with SIRT1 and Insulin Resistance
 Neurodegenerative Diseases Antibodies  >166 publications with SIRT1 and Neurodegenerative Diseases
 Liver Diseases Antibodies  >165 publications with SIRT1 and Liver Diseases
 Fatty Liver Antibodies  >138 publications with SIRT1 and Fatty Liver
 Necrosis Antibodies  >121 publications with SIRT1 and Necrosis
 Heart Diseases Antibodies  >108 publications with SIRT1 and Heart Diseases
 Atherosclerosis Antibodies  >95 publications with SIRT1 and Atherosclerosis
 Kidney Diseases Antibodies  >69 publications with SIRT1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >159 publications with SIRT1 and Brain
 Heart Antibodies  >123 publications with SIRT1 and Heart
 Adipose Tissue Antibodies  >100 publications with SIRT1 and Adipose Tissue
 Kidney Antibodies  >64 publications with SIRT1 and Kidney
 Lung Antibodies  >56 publications with SIRT1 and Lung
 Embryonic Tissue Antibodies  >31 publications with SIRT1 and Embryonic Tissue
 Prostate Antibodies  >26 publications with SIRT1 and Prostate
 Bone Marrow Antibodies  >22 publications with SIRT1 and Bone Marrow
 Eye Antibodies  >20 publications with SIRT1 and Eye
 Testis Antibodies  >7 publications with SIRT1 and Testis
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