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SRD5A2 elisa kit :: Rat steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS923230
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2), ELISA Kit

★Popular Item★
 Also Known As   

Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

 Product Synonym Names    Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA kit; MGC138457; 3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2; steroid-5-alpha-reductase; alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
 Product Gene Name   

SRD5A2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS923230 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P31214
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat SRD5A2. No significant cross-reactivity or interference between rat SRD5A2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates.
 Assay Type    Competitive
 Detection Range    6 pg/ml -1200 pg/ml.
 Sensitivity    3 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SRD5A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SRD5A2 purchase    MBS923230 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6 pg/ml -1200 pg/ml in biological research samples containing SRD5A2, with an estimated sensitivity of 3 pg/ml. The ELISA analytical biochemical technique of the MBS923230 kit is based on SRD5A2 antibody-SRD5A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRD5A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRD5A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SRD5A2 elisa kit

   Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for SRD5A2 and Horseradish Peroxidase (HRP) conjugated SRD5A2. The competitive inhibition reaction is launched between with HRP labeled SRD5A2 and unlabeled SRD5A2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SRD5A2 in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of SRD5A2 elisa kit    SRD5A2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS923230. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SRD5A2. It may not necessarily be applicable to this product.
 NCBI GI #    12083683
 NCBI GeneID    64677
 NCBI Accession #    NP_073202.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022711.4 [Other Products]
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 UniProt Primary Accession #    P31214 [Other Products]
 UniProt Related Accession #    P31214 [Other Products]
 Molecular Weight    28,772 Da
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 NCBI Official Full Name    3-oxo-5-alpha-steroid 4-dehydrogenase 2
 NCBI Official Synonym Full Names    steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
 NCBI Official Symbol    Srd5a2 [Similar Products]
 NCBI Official Synonym Symbols   
S5AR 2
[Similar Products]
 NCBI Protein Information    3-oxo-5-alpha-steroid 4-dehydrogenase 2; SR type 2; 5 alpha-SR2; steroid 5-alpha-reductase 2
 UniProt Protein Name    3-oxo-5-alpha-steroid 4-dehydrogenase 2
 UniProt Synonym Protein Names   
5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2
 Protein Family    3-oxo-5-alpha-steroid 4-dehydrogenase
 UniProt Gene Name    Srd5a2 [Similar Products]
 UniProt Synonym Gene Names    S5AR 2 [Similar Products]
 UniProt Entry Name    S5A2_RAT
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 NCBI Summary for SRD5A2    enzyme which converts testosterone to dihydrotestosterone (DHT); also acts on progesterone and corticosterone; plays a central role in sexual differentiation and androgen physiology [RGD, Feb 2006]
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 UniProt Comments for SRD5A2    Function: Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology

Catalytic activity: A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 PublicationManual assertion based on experiment in:Ref.1
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 Research Articles on SRD5A2    1. findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SRD5A2 elisa kitDiseases associated with SRD5A2 elisa kit
 Products by Pathway  Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
 Disease Name  Pubmed Publications
 Genital Diseases, Male Antibodies  >419 publications with SRD5A2 and Genital Diseases, Male
 Neoplasms Antibodies  >336 publications with SRD5A2 and Neoplasms
 Congenital Abnormalities Antibodies  >254 publications with SRD5A2 and Congenital Abnormalities
 Prostatic Neoplasms Antibodies  >246 publications with SRD5A2 and Prostatic Neoplasms
 Disorders of Sex Development Antibodies  >230 publications with SRD5A2 and Disorders of Sex Development
 Hyperplasia Antibodies  >229 publications with SRD5A2 and Hyperplasia
 Mental Disorders Antibodies  >208 publications with SRD5A2 and Mental Disorders
 Prostatic Hyperplasia Antibodies  >202 publications with SRD5A2 and Prostatic Hyperplasia
 Hirsutism Antibodies  >50 publications with SRD5A2 and Hirsutism
 Alopecia Antibodies  >46 publications with SRD5A2 and Alopecia
Organs/Tissues associated with SRD5A2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >529 publications with SRD5A2 and Prostate
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