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THPO elisa kit :: Rat thrombopoietin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702411
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
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 Product Name   

thrombopoietin (THPO), ELISA Kit

★Popular Item★
 Also Known As   

Rat Thrombopoietin, TPO ELISA Kit

 Product Synonym Names    Rat Thrombopoietin; TPO ELISA kit; MGC163194; MGDF; MKCSF; ML; MPLLG; TPO; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferativ; thrombopoietin
 Product Gene Name   

THPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702411 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P49745
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat THPO. No significant cross-reactivity or interference between Rat THPO and analogues was observed.
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 Samples    Serum, plasma
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of THPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for THPO purchase    MBS702411 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thrombopoietin (THPO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing THPO, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS702411 kit is based on THPO antibody-THPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, THPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for THPO elisa kit

   Introduction: Thrombopoietin, also known as TPO, is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. In the liver it is produced by parenchymal cells and sinusoidal endothelial cells. In the kidney it is made by proximal convoluted tubule cells. Along with these it is made by striated muscle and stromal cells in the bone marrow. In the liver, its production is augmented by interleukin 6 (IL-6). The thrombopoietin gene is located on the long arm of chromosome 3 (q26.3-27). Abnormalities in this gene occur in some hereditary forms of thrombocytosis (high platelet count) and in some cases of leukemia. Thrombopoietin shares its first 153 amino acids with erythropoietin.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TPO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of THPO elisa kit    THPO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702411. Click to request current manual
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NCBI/Uniprot data below describe general gene information for THPO. It may not necessarily be applicable to this product.
 NCBI GI #    13592113
 NCBI GeneID    81811
 NCBI Accession #    NP_112395.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031133.2 [Other Products]
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 UniProt Primary Accession #    P49745 [Other Products]
 UniProt Related Accession #    P49745 [Other Products]
 Molecular Weight    34,556 Da
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 NCBI Official Full Name    thrombopoietin
 NCBI Official Synonym Full Names    thrombopoietin
 NCBI Official Symbol    Thpo [Similar Products]
 NCBI Protein Information    thrombopoietin
 UniProt Protein Name    Thrombopoietin
 Protein Family    Thrombopoietin
 UniProt Gene Name    Thpo [Similar Products]
 UniProt Entry Name    TPO_RAT
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 NCBI Summary for THPO    lineage-specific cytokine; important for proliferation and development of megakaryocytes and platelets; may be the major regulator of circulating platelets [RGD, Feb 2006]
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 UniProt Comments for THPO    Function: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
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 Research Articles on THPO    1. The data suggest a potential role of thrombopoietin (TPO) as a physiological regulator of coronary flow. By acting on specific receptors present on endothelial cells, TPO may induce PI3K/Akt-dependent eNOS phosphorylation and NO release.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with THPO elisa kitPathways associated with THPO elisa kit
 Reference Product  PubMed Publications
 Mpl elisa kit  >23 publications with THPO and Mpl
 Kitlg elisa kit  >8 publications with THPO and Kitlg
 Epo elisa kit  >3 publications with THPO and Epo
 Bcl2l1 elisa kit  >1 publications with THPO and Bcl2l1
 Selp elisa kit  >1 publications with THPO and Selp
 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Aggregation (Plug Formation) Pathway antibodies  Platelet Aggregation (Plug Formation) Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
Diseases associated with THPO elisa kitOrgans/Tissues associated with THPO elisa kit
 Disease Name  Pubmed Publications
 Thrombocytosis Antibodies  >9 publications with THPO and Thrombocytosis
 Anemia Antibodies  >5 publications with THPO and Anemia
 Cardiovascular Diseases Antibodies  >5 publications with THPO and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >3 publications with THPO and Lung Neoplasms
 Congenital Abnormalities Antibodies  >2 publications with THPO and Congenital Abnormalities
 Carcinoma, Squamous Cell Antibodies  >2 publications with THPO and Carcinoma, Squamous Cell
 Pain Antibodies  >2 publications with THPO and Pain
 Thrombocythemia, Essential Antibodies  >2 publications with THPO and Thrombocythemia, Essential
 Precancerous Conditions Antibodies  >1 publications with THPO and Precancerous Conditions
 Hypertension Antibodies  >1 publications with THPO and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >39 publications with THPO and Brain
 Kidney Antibodies  >7 publications with THPO and Kidney
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