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TCN2 elisa kit :: Rat transcobalamin II; macrocytic anemia ELISA Kit

Scan QR to view Datasheet Catalog #    MBS928945 TCN2 elisa kit
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

transcobalamin II; macrocytic anemia (TCN2), ELISA Kit

 Also Known As   

Rat Transcobalamin-2, TCN2 ELISA Kit

 Product Synonym Names    Rat Transcobalamin-2 (TCN2) ELISA kit; D22S676; D22S750; TC2; transcobalamin II; transcobalamin II; macrocytic anemia
 Product Gene Name   

TCN2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9R0D6
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat TCN2. No significant cross-reactivity or interference between Rat TCN2 and analogues was observed.
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 Assay Type    Sandwich
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transport
 Protein Biological Process 2    Anion transport
 Protein Biological Process 3    Cobalt transport
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TCN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TCN2 purchase    MBS928945 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transcobalamin II; macrocytic anemia (TCN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TCN2. The ELISA analytical biochemical technique of the MBS928945 kit is based on TCN2 antibody-TCN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TCN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TCN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TCN2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS928945. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
 NCBI GI #    11968124
 NCBI GeneID    64365
 NCBI Accession #    NP_071979.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022534.1 [Other Products]
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 UniProt Primary Accession #    Q9R0D6 [Other Products]
 UniProt Related Accession #    Q9R0D6 [Other Products]
 Molecular Weight    47,420 Da
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 NCBI Official Full Name    transcobalamin-2
 NCBI Official Synonym Full Names    transcobalamin 2
 NCBI Official Symbol    Tcn2 [Similar Products]
 NCBI Official Synonym Symbols   
Tc2; Tcn2p
[Similar Products]
 NCBI Protein Information    transcobalamin-2; TC-2; TCII; TC II; transcobalamin II
 UniProt Protein Name    Transcobalamin-2
 UniProt Synonym Protein Names   
Transcobalamin II; TC II; TCII
 Protein Family    Transcobalamin
 UniProt Gene Name    Tcn2 [Similar Products]
 UniProt Synonym Gene Names    TC-2; TC II; TCII [Similar Products]
 UniProt Entry Name    TCO2_RAT
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 NCBI Summary for TCN2    component of plasma cobalamin (Cbl; vitamin B12) transport to all cells [RGD, Feb 2006]
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 UniProt Comments for TCN2    TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space

Molecular Function: metal ion binding; cobalamin binding

Biological Process: cobalamin metabolic process; cobalamin transport; cobalt ion transport
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TCN2 elisa kitDiseases associated with TCN2 elisa kit
 Products by Pathway  Pathway Diagram
 One Carbon Metabolism Pathway antibodies  One Carbon Metabolism Pathway Diagram
 Vitamin Digestion And Absorption Pathway antibodies  Vitamin Digestion And Absorption Pathway Diagram
 Vitamin Digestion And Absorption Pathway antibodies  Vitamin Digestion And Absorption Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with TCN2 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >6 publications with TCN2 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >5 publications with TCN2 and Vascular Diseases
 Anemia Antibodies  >4 publications with TCN2 and Anemia
 Brain Diseases Antibodies  >4 publications with TCN2 and Brain Diseases
 Anemia, Megaloblastic Antibodies  >4 publications with TCN2 and Anemia, Megaloblastic
 Cleft Lip Antibodies  >3 publications with TCN2 and Cleft Lip
 Anemia, Macrocytic Antibodies  >3 publications with TCN2 and Anemia, Macrocytic
 Neurologic Manifestations Antibodies  >2 publications with TCN2 and Neurologic Manifestations
Organs/Tissues associated with TCN2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with TCN2 and Brain
 Heart Antibodies  >2 publications with TCN2 and Heart
 Kidney Antibodies  >2 publications with TCN2 and Kidney
 Prostate Antibodies  >1 publications with TCN2 and Prostate
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