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TGFB2 elisa kit :: Rat transforming growth factor, beta 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702095
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,300 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,340 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

transforming growth factor, beta 2 (TGFB2), ELISA Kit

 Also Known As   

Rat Transforming Growth factor beta2, TGF-beta2 ELISA Kit

 Product Synonym Names    Rat Transforming Growth factor beta2; TGF-beta2 ELISA kit; MGC116892; TGF-beta2; ; transforming growth factor; beta 2
 Product Gene Name   

TGFB2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702095 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q07257
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat TGFB2. No significant cross-reactivity or interference between Rat TGFB2 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    3.9 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFB2 purchase    MBS702095 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the transforming growth factor, beta 2 (TGFB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing TGFB2, with an estimated sensitivity of 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS702095 kit is based on TGFB2 antibody-TGFB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TGFB2 elisa kit

   Introduction: Transforming growth factor beta (TGF-beta) is a protein that comes in three isoforms called TGF-beta1, TGF-beta2 and TGF-beta3; it was also the original name for the founding member of this family that is now called TGF-beta1. The TGF-beta family is part of a superfamily of proteins known as the transforming growth factor beta superfamily, which includes inhibins, activin, anti-müllerian hormone, bone morphogenetic protein, decapentaplegic and Vg-1. TGF beta controls proliferation, differentiation, and other functions in most cell types. It can also act as a negative autocrine growth factor. TGF-beta2 is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. There are two named isoforms of this protein, created by alternative splicing of the same gene.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TGFB2 elisa kit    TGFB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702095. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFB2. It may not necessarily be applicable to this product.
 NCBI GI #    13592109
 NCBI GeneID    81809
 NCBI Accession #    NP_112393.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031131.1 [Other Products]
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 UniProt Primary Accession #    Q07257 [Other Products]
 UniProt Secondary Accession #    Q63574; Q9QW26; Q9R281; Q9R298; Q9R2B8; Q9WUQ8 [Other Products]
 UniProt Related Accession #    Q07257 [Other Products]
 Molecular Weight    50,534 Da
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 NCBI Official Full Name    transforming growth factor beta-2
 NCBI Official Synonym Full Names    transforming growth factor, beta 2
 NCBI Official Symbol    Tgfb2 [Similar Products]
 NCBI Official Synonym Symbols   
TGF-B2
[Similar Products]
 NCBI Protein Information    transforming growth factor beta-2; TGF-beta-2; TGF beta 2 protein
 UniProt Protein Name    Transforming growth factor beta-2
 Protein Family    Transforming growth factor
 UniProt Gene Name    Tgfb2 [Similar Products]
 UniProt Synonym Gene Names    TGF-beta-2; LAP [Similar Products]
 UniProt Entry Name    TGFB2_RAT
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 NCBI Summary for TGFB2    binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]
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 UniProt Comments for TGFB2    Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
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 Research Articles on TGFB2    1. Transforming growth factor-beta2 and -beta3 potently decreased basal Sema3A expression; the maximum effect was at very low levels and completely cancelled the activities of Fibroblast Growth Factor 2 and Hepatocyte Growth Factor to upregulate Sema3A.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFB2 elisa kitPathways associated with TGFB2 elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >931 publications with TGFB2 and TGFB1
 TGFB3 elisa kit  >512 publications with TGFB2 and TGFB3
 CTGF elisa kit  >44 publications with TGFB2 and CTGF
 TGFBR3 elisa kit  >43 publications with TGFB2 and TGFBR3
 BMP2 elisa kit  >21 publications with TGFB2 and BMP2
 TGFBR2 elisa kit  >16 publications with TGFB2 and TGFBR2
 ENG elisa kit  >11 publications with TGFB2 and ENG
 TGFBR1 elisa kit  >9 publications with TGFB2 and TGFBR1
 APP elisa kit  >5 publications with TGFB2 and APP
 LTBP3 elisa kit  >1 publications with TGFB2 and LTBP3
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
 Colorectal Cancer Pathway antibodies  Colorectal Cancer Pathway Diagram
Diseases associated with TGFB2 elisa kitOrgans/Tissues associated with TGFB2 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >270 publications with TGFB2 and Fibrosis
 Inflammation Antibodies  >177 publications with TGFB2 and Inflammation
 Necrosis Antibodies  >171 publications with TGFB2 and Necrosis
 Nervous System Diseases Antibodies  >120 publications with TGFB2 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >117 publications with TGFB2 and Cardiovascular Diseases
 Glaucoma Antibodies  >104 publications with TGFB2 and Glaucoma
 Heart Diseases Antibodies  >57 publications with TGFB2 and Heart Diseases
 Breast Neoplasms Antibodies  >55 publications with TGFB2 and Breast Neoplasms
 Liver Diseases Antibodies  >53 publications with TGFB2 and Liver Diseases
 Kidney Diseases Antibodies  >51 publications with TGFB2 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >356 publications with TGFB2 and Eye
 Bone Antibodies  >260 publications with TGFB2 and Bone
 Muscle Antibodies  >229 publications with TGFB2 and Muscle
 Vascular Antibodies  >213 publications with TGFB2 and Vascular
 Connective Tissue Antibodies  >184 publications with TGFB2 and Connective Tissue
 Embryonic Tissue Antibodies  >140 publications with TGFB2 and Embryonic Tissue
 Brain Antibodies  >134 publications with TGFB2 and Brain
 Skin Antibodies  >131 publications with TGFB2 and Skin
 Lung Antibodies  >128 publications with TGFB2 and Lung
 Heart Antibodies  >84 publications with TGFB2 and Heart
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