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TH elisa kit :: Rat tyrosine hydroxylase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701326
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tyrosine hydroxylase (TH), ELISA Kit

★Popular Item★
 Also Known As   

Rat Tyrosine Hydroxylase, TH ELISA Kit

 Product Synonym Names    Rat Tyrosine Hydroxylase (TH) ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; tyrosine hydroxylase
 Product Gene Name   

TH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701326 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P04177
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat TH. No significant cross-reactivity or interference between rat TH and analogues was observed.
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 Samples    Serum, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    78 pg/ml -5000 pg/ml.
 Sensitivity    19.5 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TH purchase    MBS701326 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tyrosine hydroxylase (TH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78 pg/ml -5000 pg/ml in biological research samples containing TH, with an estimated sensitivity of 19.5 pg/ml. The ELISA analytical biochemical technique of the MBS701326 kit is based on TH antibody-TH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TH elisa kit

   Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TH elisa kit    TH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701326. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.
 NCBI GI #    6981652
 NCBI GeneID    25085
 NCBI Accession #    NP_036872.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012740.3 [Other Products]
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 UniProt Primary Accession #    P04177 [Other Products]
 UniProt Related Accession #    P04177 [Other Products]
 Molecular Weight    55,966 Da
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 NCBI Official Full Name    tyrosine 3-monooxygenase
 NCBI Official Synonym Full Names    tyrosine hydroxylase
 NCBI Official Symbol    Th [Similar Products]
 NCBI Official Synonym Symbols   
The
[Similar Products]
 NCBI Protein Information    tyrosine 3-monooxygenase; tyrosine 3-hydroxylase
 UniProt Protein Name    Tyrosine 3-monooxygenase
 UniProt Synonym Protein Names   
Tyrosine 3-hydroxylase; TH
 UniProt Gene Name    Th [Similar Products]
 UniProt Synonym Gene Names    TH [Similar Products]
 UniProt Entry Name    TY3H_RAT
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 NCBI Summary for TH    catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]
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 UniProt Comments for TH    Function: Plays an important role in the physiology of adrenergic neurons.

Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactor: Fe2+ ion.

Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity.
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 Research Articles on TH    1. there exists possible interrelation between TH and tryptophan hydroxylase gene expression and epigenetic histone acetylation in chronic unpredictable stress-induced depressive rats, which at least partly contributes to the etiology of depression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TH elisa kitPathways associated with TH elisa kit
 Reference Product  PubMed Publications
 MAPK3 elisa kit  >1186 publications with TH and MAPK3
 MAPK1 elisa kit  >1132 publications with TH and MAPK1
 SNCA elisa kit  >101 publications with TH and SNCA
 MAPKAPK2 elisa kit  >56 publications with TH and MAPKAPK2
 YWHAZ elisa kit  >7 publications with TH and YWHAZ
 CAMK2G elisa kit  >5 publications with TH and CAMK2G
 YWHAB elisa kit  >4 publications with TH and YWHAB
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Synthesis Pathway antibodies  Catecholamine Synthesis Pathway Diagram
Diseases associated with TH elisa kitOrgans/Tissues associated with TH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >895137 publications with TH and Nervous System Diseases
 Heart Diseases Antibodies  >491595 publications with TH and Heart Diseases
 Brain Diseases Antibodies  >428916 publications with TH and Brain Diseases
 Pain Antibodies  >330424 publications with TH and Pain
 Kidney Diseases Antibodies  >213722 publications with TH and Kidney Diseases
 Inflammation Antibodies  >179411 publications with TH and Inflammation
 Hypertension Antibodies  >176651 publications with TH and Hypertension
 Necrosis Antibodies  >114971 publications with TH and Necrosis
 Myocardial Infarction Antibodies  >106797 publications with TH and Myocardial Infarction
 Arrhythmias, Cardiac Antibodies  >97981 publications with TH and Arrhythmias, Cardiac
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >250535 publications with TH and Brain
 Eye Antibodies  >133206 publications with TH and Eye
 Nerve Antibodies  >114125 publications with TH and Nerve
 Mouth Antibodies  >96016 publications with TH and Mouth
 Pancreas Antibodies  >37955 publications with TH and Pancreas
 Adrenal Gland Antibodies  >18619 publications with TH and Adrenal Gland
 Embryonic Tissue Antibodies  >15088 publications with TH and Embryonic Tissue
 Placenta Antibodies  >8218 publications with TH and Placenta
 Uncharacterized Tissue Antibodies  >86 publications with TH and Uncharacterized Tissue
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