Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow VIM rightarrow Rat VIM  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Total Immunoglobulin (TIG) ELISA Kit
 • Hepatitis C Virus (HCV) NS-5a Region (a.a. 2190-2300) Antibody
 • Trifunctional enzyme subunit alpha, mitochondrial (Hadha) Recombinant Protein
 • RTKN Antibody
 • Somatostatin (SST) ELISA Kit
 • CD34 Antibody
 • hnRNP H Antibody
 • Cytokeratin 10 (KRT10) Antibody
 • Insulin Like Growth Factor 1 (IGF1) ELISA Kit
 • Bit1 (PTRH2) Antibody
 • RSRC1 cDNA Clone
 • Tafazzin (TAZ) Antibody
 • Heart Fatty Acid Binding Protein (H-FABP) ELISA Kit
 • somatostatin (SST) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

VIM elisa kit :: Rat vimentin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702882
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

vimentin, ELISA Kit

★Popular Item★
 Also Known As   

Rat vimentin, VIM ELISA Kit

 Product Synonym Names    Rat vimentin (VIM) ELISA Kit; RP11-124N14.1; FLJ36605; ; vimentin
 Product Gene Name   

VIM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702882 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P31000
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of rat VIM. No significant cross-reactivity or interference between rat VIM and analogues was observed.
Section Bar
 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    23.5 ng/ml-1500 ng/ml.
 Sensitivity    The minimum detectable dose of rat VIM is typically less than 5.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Section Bar
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VIM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VIM purchase    MBS702882 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vimentin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 23.5 ng/ml-1500 ng/ml in biological research samples containing VIM, with an estimated sensitivity of The minimum detectable dose of rat VIM is typically less than 5.8 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS702882 kit is based on VIM antibody-VIM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VIM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VIM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for VIM elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VIM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of VIM elisa kit    VIM elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS702882. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.
 NCBI GI #    14389299
 NCBI GeneID    81818
 NCBI Accession #    NP_112402.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031140.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P31000 [Other Products]
 UniProt Related Accession #    P31000 [Other Products]
 Molecular Weight    53,733 Da
Table BarTOPTable Bar
 NCBI Official Full Name    vimentin
 NCBI Official Synonym Full Names    vimentin
 NCBI Official Symbol    Vim [Similar Products]
 NCBI Protein Information    vimentin
 UniProt Protein Name    Vimentin
 Protein Family    Vimentin
 UniProt Gene Name    Vim [Similar Products]
 UniProt Entry Name    VIME_RAT
Section Bar
 NCBI Summary for VIM    class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006]
Section Bar
 UniProt Comments for VIM    Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 PublicationManual assertion based on experiment in:Ref.6
Table BarTOPTable Bar
 Research Articles on VIM    1. TGFbeta stimulates vimentin production via PI3K-Akt-mTOR signaling, which leads to suppression of ATF4-dependent Ocn transcription and osteoblast differentiation.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with VIM elisa kitPathways associated with VIM elisa kit
 Reference Product  PubMed Publications
 Des elisa kit  >38 publications with VIM and Des
 Cdh2 elisa kit  >9 publications with VIM and Cdh2
 Dmd elisa kit  >1 publications with VIM and Dmd
 Casp8 elisa kit  >1 publications with VIM and Casp8
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with VIM elisa kitOrgans/Tissues associated with VIM elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >407 publications with VIM and Nervous System Diseases
 Neurodegenerative Diseases Antibodies  >175 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >51 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >39 publications with VIM and Kidney Diseases
 Pain Antibodies  >36 publications with VIM and Pain
 Disease Models, Animal Antibodies  >36 publications with VIM and Disease Models, Animal
 Hemorrhage Antibodies  >34 publications with VIM and Hemorrhage
 Neoplasm Metastasis Antibodies  >32 publications with VIM and Neoplasm Metastasis
 Liver Diseases Antibodies  >32 publications with VIM and Liver Diseases
 Breast Neoplasms Antibodies  >29 publications with VIM and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >408 publications with VIM and Brain
 Blood Antibodies  >169 publications with VIM and Blood
 Muscle Antibodies  >93 publications with VIM and Muscle
 Nerve Antibodies  >65 publications with VIM and Nerve
 Bone Antibodies  >64 publications with VIM and Bone
 Kidney Antibodies  >48 publications with VIM and Kidney
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Liver Antibodies  >42 publications with VIM and Liver
 Vascular Antibodies  >35 publications with VIM and Vascular
 Eye Antibodies  >32 publications with VIM and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions