Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ADAM8 rightarrow Sheep ADAM8  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • SLC2A4 Antibody
 • Insulin-degrading enzyme (IDE) ELISA Kit
 • Protein Kinase A, beta, Catalytic Subunit (PRKACB) Antibody
 • Chlamydia trachomatis (CT) antibody (IgG) ELISA Kit
 • A-G-specific adenine DNA glycosylase (MUTYH) Antibody
 • CD79a-CD5 Dual Color Reagent
 • arginine vasopressin (AVP) ELISA Kit
 • SEL1L Peptide
 • Adrenocorticotropic Hormone Receptor (ACTHR) ELISA Kit
 • VEGF Receptor 1 (FLT1) Antibody
 • Neurofibromin (paired S2741) (NF1) Antibody
 • Glucagon Like Peptide 2 (GLP2) ELISA Kit
 • Gamma Aminobutyric Acid (GABA) ELISA Kit
 • soluble fibrin monomer complex, SFMC ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ADAM8 elisa kit :: Sheep A Disintegrin And Metalloprotease 8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740383
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

A Disintegrin And Metalloprotease 8 (ADAM8), ELISA Kit

 Also Known As   

Sheep A Disintegrin And Metalloprotease 8 ELISA Kit

 Product Gene Name   

ADAM8 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602267
Section Bar
 Species Reactivity    Sheep
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL
 Intended Uses    This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ADAM8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADAM8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ADAM8 purchase    MBS740383 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM8, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740383 kit is based on ADAM8 antibody-ADAM8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ADAM8 elisa kit

   Principle of the Assay: ADAM8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM8 antibody and an ADAM8-HRP conjugate. The assay sample and buffer are incubated together with ADAM8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM8 concentration since ADAM8 from samples and ADAM8-HRP conjugate compete for the anti-ADAM8 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM8 from the sample, fewer sites are left to bind ADAM8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM8 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ADAM8 elisa kit    Cardiovascular
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ADAM8 elisa kit    ADAM8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS740383. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ADAM8. It may not necessarily be applicable to this product.
 NCBI GI #    109731932
 NCBI GeneID    101
 NCBI Accession #    AAI15405.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    B4DVM6; H0YL36; H0YLR0; H0YN39 [Other Products]
 UniProt Related Accession #    P78325 [Other Products]
 Molecular Weight    80,262 Da
Table BarTOPTable Bar
 NCBI Official Full Name    ADAM8 protein
 NCBI Official Synonym Full Names    ADAM metallopeptidase domain 8
 NCBI Official Symbol    ADAM8 [Similar Products]
 NCBI Official Synonym Symbols   
MS2; CD156; CD156a
[Similar Products]
 NCBI Protein Information    disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8
 UniProt Protein Name    Disintegrin and metalloproteinase domain-containing protein 8
 UniProt Synonym Protein Names   
Cell surface antigen MS2; CD_antigen: CD156a
 Protein Family    Disintegrin and metalloproteinase domain-containing protein
 UniProt Gene Name    ADAM8 [Similar Products]
 UniProt Synonym Gene Names    MS2; ADAM 8 [Similar Products]
 UniProt Entry Name    ADAM8_HUMAN
Section Bar
 NCBI Summary for ADAM8    This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]
Section Bar
 UniProt Comments for ADAM8    ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; cell adhesion molecule binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; positive regulation of protein kinase B signaling cascade; extracellular matrix disassembly; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; lymphocyte chemotaxis
Table BarTOPTable Bar
 Research Articles on ADAM8    1. Data show that Cu2+-generated reactive oxygen species (ROS), human ADAM8, ADAM10, and ADAM17 are all capable of cleaving mouse PrP (MoPrP).
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with ADAM8 elisa kitDiseases associated with ADAM8 elisa kit
 Products by Pathway  Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with ADAM8 and Inflammation
 Lung Diseases Antibodies  >20 publications with ADAM8 and Lung Diseases
 Necrosis Antibodies  >18 publications with ADAM8 and Necrosis
 Disease Models, Animal Antibodies  >13 publications with ADAM8 and Disease Models, Animal
 Brain Diseases Antibodies  >8 publications with ADAM8 and Brain Diseases
 Cardiovascular Diseases Antibodies  >8 publications with ADAM8 and Cardiovascular Diseases
 Fibrosis Antibodies  >6 publications with ADAM8 and Fibrosis
 Liver Diseases Antibodies  >6 publications with ADAM8 and Liver Diseases
 Carcinoma, Squamous Cell Antibodies  >6 publications with ADAM8 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >6 publications with ADAM8 and Lung Neoplasms
Organs/Tissues associated with ADAM8 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with ADAM8 and Blood
 Lung Antibodies  >22 publications with ADAM8 and Lung
 Brain Antibodies  >16 publications with ADAM8 and Brain
 Connective Tissue Antibodies  >7 publications with ADAM8 and Connective Tissue
 Kidney Antibodies  >6 publications with ADAM8 and Kidney
 Bone Marrow Antibodies  >6 publications with ADAM8 and Bone Marrow
 Stomach Antibodies  >5 publications with ADAM8 and Stomach
 Eye Antibodies  >2 publications with ADAM8 and Eye
 Spleen Antibodies  >2 publications with ADAM8 and Spleen
 Prostate Antibodies  >2 publications with ADAM8 and Prostate
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions