Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow AGC rightarrow Sheep AGC  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • GPR25 Antibody
 • neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) ELISA Kit
 • L-lactate dehydrogenase (LDH) Recombinant Protein
 • CD184 (pS339) (CXCR4) Antibody
 • Serum Amyloid A (SAA) ELISA Kit
 • S100 Antibody
 • C20orf75 (LRRN4) Peptide
 • phospholipase C-like 1 (PLCL1) ELISA Kit
 • CDC5L Antibody
 • choline-ethanolamine phosphotransferase 1 (CEPT1) Antibody
 • PLC gamma1 Antibody
 • CD66e Antibody
 • KLRG1 Antibody
 • killer cell immunoglobulin-like receptor, two domains, long ... ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

AGC elisa kit :: Sheep AGC ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2509778
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $585 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AGC, ELISA Kit

 Also Known As   

Sheep AGC (Aggrecan) ELISA Kit

 Product Gene Name   

AGC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q61282
 Species Reactivity    Sheep
Section Bar
 Specificity    This kit recognizes natural and recombinant Sheep AGC. No significant cross-reactivity or interference between Sheep AGC and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.781-50ng/mL
 Sensitivity    Min: 0.469ng/mL; Max: 50ng/mL
Section Bar
 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AGC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AGC purchase    MBS2509778 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AGC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing AGC, with an estimated sensitivity of Min: 0.469ng/mL; Max: 50ng/mL. The ELISA analytical biochemical technique of the MBS2509778 kit is based on AGC antibody-AGC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for AGC elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep AGC concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AGC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AGC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AGC. You can calculate the concentration of AGC in the samples by comparing the OD of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of AGC elisa kit    AGC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2509778. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for AGC. It may not necessarily be applicable to this product.
 NCBI GI #    116875858
 NCBI GeneID    11595
 NCBI Accession #    NP_031450.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007424.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q61282 [Other Products]
 UniProt Secondary Accession #    Q64021; E9QLS9 [Other Products]
 UniProt Related Accession #    Q61282 [Other Products]
 Molecular Weight    221,941 Da
Table BarTOPTable Bar
 NCBI Official Full Name    aggrecan core protein
 NCBI Official Synonym Full Names    aggrecan
 NCBI Official Symbol    Acan [Similar Products]
 NCBI Official Synonym Symbols   
Agc; cmd; Agc1; Cspg1; b2b183Clo
[Similar Products]
 NCBI Protein Information    aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein
 UniProt Protein Name    Aggrecan core protein
 UniProt Synonym Protein Names   
Cartilage-specific proteoglycan core protein; CSPCP
 UniProt Gene Name    Acan [Similar Products]
 UniProt Synonym Gene Names    Agc; Agc1; CSPCP [Similar Products]
 UniProt Entry Name    PGCA_MOUSE
Section Bar
 UniProt Comments for AGC    ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Cell adhesion

Cellular Component: proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: collagen fibril organization; central nervous system development; proteoglycan biosynthetic process; chondrocyte development; cell adhesion; negative regulation of cell migration; cartilage condensation
Table BarTOPTable Bar
 Research Articles on AGC    1. Aggrecan, link protein and TN-R were identified to be essential for the neuroprotective properties of the perineuronal net.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with AGC elisa kitPathways associated with AGC elisa kit
 Reference Product  PubMed Publications
 Adamts5 elisa kit  >14 publications with AGC and Adamts5
 Adamts4 elisa kit  >9 publications with AGC and Adamts4
 Lum elisa kit  >3 publications with AGC and Lum
 Products by Pathway  Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
Diseases associated with AGC elisa kitOrgans/Tissues associated with AGC elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with AGC and Necrosis
 Pain Antibodies  >7 publications with AGC and Pain
 Inflammation Antibodies  >7 publications with AGC and Inflammation
 Arthritis, Rheumatoid Antibodies  >4 publications with AGC and Arthritis, Rheumatoid
 Adenoma Antibodies  >3 publications with AGC and Adenoma
 Weight Loss Antibodies  >2 publications with AGC and Weight Loss
 Hyperplasia Antibodies  >1 publications with AGC and Hyperplasia
 Fibrosis Antibodies  >1 publications with AGC and Fibrosis
 Liver Diseases Antibodies  >1 publications with AGC and Liver Diseases
 Spondyloepiphyseal Dysplasia, Kimberley Type Antibodies  >1 publications with AGC and Spondyloepiphyseal Dysplasia, Kimberley Type
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with AGC and Brain
 Embryonic Tissue Antibodies  >11 publications with AGC and Embryonic Tissue
 Skin Antibodies  >4 publications with AGC and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions