Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow DEFbeta2 rightarrow Sheep DEFbeta2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Endoglucanase Recombinant Protein
 • R-spondin 2 homolog (Xenopus laevis) (RSPO2) ELISA Kit
 • Dnak Recombinant Protein
 • NFkappaB-p65 Antibody
 • complement factor H (CFH) ELISA Kit
 • N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit
 • AIFM1 Recombinant Protein
 • aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2) Antibody
 • M13, fd, F1 Bacteriophages, Filamentous Antibody
 • Influenza A virus Nucleoprotein (NP) Recombinant Protein
 • Elastase 1, Pancreatic (ELA1) ELISA Kit
 • 9 alpha, 11 beta PGF2 alpha (9alpha, 11beta PGF2alpha) ELISA Kit
 • EIPS2 ELISA Kit
 • STAMBP Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

DEFbeta2 elisa kit :: Sheep Defensin Beta 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS028880 DEFbeta2 elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Defensin Beta 2 (DEFbeta2), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Defensin Beta 2 ELISA Kit

 Product Gene Name   

DEFbeta2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 8; NC_000074.6 (21839926..21843482). Location: 8 A4; 8 10.45 cM
 3D Structure    ModBase 3D Structure for P82020
Section Bar
 Species Reactivity    Sheep
Section Bar
 Specificity    No significant cross-reactivity or interference between Sheep DEF?2 and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml - 500 pg/ml.
 Sensitivity    2.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEF?2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep DEF?2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEF?2 and DEF?2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEF?2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFbeta2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFbeta2 purchase    MBS028880 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 2 (DEFbeta2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml - 500 pg/ml in biological research samples containing DEFbeta2, with an estimated sensitivity of 2.0 pg/ml. The ELISA analytical biochemical technique of the MBS028880 kit is based on DEFbeta2 antibody-DEFbeta2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFbeta2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFbeta2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for DEFbeta2 elisa kit

   Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEF?2 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep DEF?2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEF?2 and DEF?2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEF?2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS028880. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for DEFbeta2. It may not necessarily be applicable to this product.
 NCBI GI #    80478968
 NCBI GeneID    13215
 NCBI Accession #    AAI09130.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P82020 [Other Products]
 UniProt Related Accession #    P82020 [Other Products]
 Molecular Weight    7,820 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Defensin beta 2
 NCBI Official Synonym Full Names    defensin beta 2
 NCBI Official Symbol    Defb2 [Similar Products]
 NCBI Official Synonym Symbols   
BD-2
[Similar Products]
 NCBI Protein Information    beta-defensin 2
 UniProt Protein Name    Beta-defensin 2
 UniProt Synonym Protein Names   
Defensin, beta 2
 UniProt Gene Name    Defb2 [Similar Products]
 UniProt Synonym Gene Names    BD-2; mBD-2 [Similar Products]
 UniProt Entry Name    DEFB2_MOUSE
Section Bar
 UniProt Comments for DEFbeta2    Function: Has bactericidal activity

By similarity.

Subcellular location: Secreted

By similarity.

Tissue specificity: Kidney, uterus and to a lesser extent in heart. Ref.1

Induction: In tracheal epithelium, by lipopolysaccharide or inflammation. Ref.1

Sequence similarities: Belongs to the beta-defensin family.
Table BarTOPTable Bar
 Research Articles on DEFbeta2    1. studied the time course of TLR9 and BD-2gene expression by the corneal epithelial cells in herpetic keratitis in C57Bl/6 and BALB/c mice
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with DEFbeta2 elisa kitDiseases associated with DEFbeta2 elisa kit
 Reference Product  PubMed Publications
 Ccr6 elisa kit  >2 publications with DEFbeta2 and Ccr6
 Defb3 elisa kit  >1 publications with DEFbeta2 and Defb3
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >4 publications with DEFbeta2 and Necrosis
 Intestinal Diseases Antibodies  >3 publications with DEFbeta2 and Intestinal Diseases
 Inflammation Antibodies  >2 publications with DEFbeta2 and Inflammation
 Metaplasia Antibodies  >1 publications with DEFbeta2 and Metaplasia
Organs/Tissues associated with DEFbeta2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >1 publications with DEFbeta2 and Epididymis
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions