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E2 elisa kit :: Sheep Estradiol (E2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702098 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $370 +1 FREE 8GB USB
96-Strip-Wells  /  $525 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,250 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
 
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 Product Name   

Estradiol (E2), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Estradiol, E2 ELISA Kit

 Product Synonym Names    Sheep Estradiol (E2) ELISA Kit; Sheep Estradiol (E2)
 Product Gene Name   

E2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702098 Testing Data    Testing Data PDF
 Species Reactivity    Sheep
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    25 pg/ml-1000 pg/ml
 Sensitivity    25 pg/ml
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Sex hormone
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of E2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for E2 purchase    MBS702098 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estradiol (E2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml-1000 pg/ml in biological research samples containing E2, with an estimated sensitivity of 25 pg/ml. The ELISA analytical biochemical technique of the MBS702098 kit is based on E2 antibody-E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect E2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, E2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for E2 elisa kit

   Introduction: Estradiol (E2 or 17beta-estradiol) (also oestradiol) is a sex hormone. During the reproductive years, most estradiol in women is produced by the granulosa cells of the ovaries by the aromatization of androstenedione (produced in the theca folliculi cells) to estrone, followed by conversion of estrone to estradiol by 17beta-hydroxysteroid reductase. Smaller amounts of estradiol are also produced by the adrenal cortex, and (in men), by the testes. Estradiol is not only produced in the gonads: in both sexes, precursor hormones, specifically testosterone, are converted by aromatization to estradiol. In particular, fat cells are active to convert precursors to estradiol, and will continue to do so even after menopause. Estradiol is also produced in the brain and in arterial walls. Estradiol enters cells freely and interacts with a cytoplasmic target cell receptor. When the estrogen receptor has bound its ligand it can enter the nucleus of the target cell, and regulate gene transcription which leads to formation of messenger RNA. The mRNA interacts with ribosomes to produce specific proteins that express the effect of estradiol upon the target cell. Estradiol binds well to both estrogen receptors, ERalpha and ERbeta, in contrast to certain other estrogens, notably medications that preferentially act on one of these receptors. These medications are called selective estrogen receptor modulators, or SERMs.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated Estradiol and antibody preparation specific for Estradiol and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm } 2 nm. The concentration of Estradiol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of E2 elisa kit    E2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702098. Click to request current manual
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 Protein Family    Regulatory protein
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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