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HP elisa kit :: Sheep Haptoglobin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7606798
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Haptoglobin (HP), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Haptoglobin ELISA Kit

 Product Gene Name   

HP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS7606798 COA    COA PDF
 OMIM    K00422 mRNA
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 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of HP. No significant crossreactivity or interference between HP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    15.625-1000ng/ml
 Sensitivity    <9.375ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HP purchase    MBS7606798 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Haptoglobin (HP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000ng/ml in biological research samples containing HP, with an estimated sensitivity of <9.375ng/ml. The ELISA analytical biochemical technique of the MBS7606798 kit is based on HP antibody-HP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HP elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HP can be calculated.
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 Typical Standard Curve/Testing Data of HP elisa kit    HP elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7606798. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HP. It may not necessarily be applicable to this product.
 NCBI GI #    386783
 NCBI GeneID    3240
 NCBI Accession #    AAA88080.1 [Other Products]
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 UniProt Secondary Accession #    P00737; Q0VAC4; Q0VAC5; Q2PP15; Q3B7J0; Q6LBY9; Q9UC67; B0AZL5 [Other Products]
 UniProt Related Accession #    P00738 [Other Products]
 Molecular Weight    38,452 Da [Similar Products]
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 NCBI Official Full Name    haptoglobin
 NCBI Official Synonym Full Names    haptoglobin
 NCBI Official Symbol    HP [Similar Products]
 NCBI Official Synonym Symbols   
BP; HPA1S; HP2ALPHA2
[Similar Products]
 NCBI Protein Information    haptoglobin
 UniProt Protein Name    Haptoglobin
 UniProt Synonym Protein Names   
Zonulin
 Protein Family    Haptoglobin
 UniProt Gene Name    HP [Similar Products]
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 NCBI Summary for HP    This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
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 UniProt Comments for HP    HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Endoplasmic reticulum; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular region; extracellular space

Molecular Function: hemoglobin binding; protein binding

Biological Process: defense response; negative regulation of oxidoreductase activity; neutrophil degranulation; receptor-mediated endocytosis; response to hydrogen peroxide

Disease: Anhaptoglobinemia
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 Research Articles on HP    1. Our findings suggest that increased serum zonulin concentration, i.e., increased intestinal permeability, contributes to metabolic endotoxemia, systemic inflammation, and insulin resistance in overweight pregnant women
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HP elisa kitPathways associated with HP elisa kit
 Reference Product  PubMed Publications
 CD163 elisa kit  >54 publications with HP and CD163
 ALB elisa kit  >39 publications with HP and ALB
 APOE elisa kit  >23 publications with HP and APOE
 IL6 elisa kit  >22 publications with HP and IL6
 APOA1 elisa kit  >9 publications with HP and APOA1
 HBB elisa kit  >4 publications with HP and HBB
 MIS12 elisa kit  >2 publications with HP and MIS12
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Scavenging Of Heme From Plasma Pathway antibodies  Scavenging Of Heme From Plasma Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with HP elisa kitOrgans/Tissues associated with HP elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >1375 publications with HP and Cardiovascular Diseases
 Inflammation Antibodies  >926 publications with HP and Inflammation
 Heart Diseases Antibodies  >612 publications with HP and Heart Diseases
 Hypersensitivity Antibodies  >585 publications with HP and Hypersensitivity
 Liver Diseases Antibodies  >489 publications with HP and Liver Diseases
 Kidney Diseases Antibodies  >411 publications with HP and Kidney Diseases
 Drug Toxicity Antibodies  >392 publications with HP and Drug Toxicity
 Necrosis Antibodies  >386 publications with HP and Necrosis
 Hypertension Antibodies  >377 publications with HP and Hypertension
 Autoimmune Diseases Antibodies  >373 publications with HP and Autoimmune Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4899 publications with HP and Blood
 Stomach Antibodies  >1227 publications with HP and Stomach
 Liver Antibodies  >1042 publications with HP and Liver
 Lung Antibodies  >941 publications with HP and Lung
 Muscle Antibodies  >630 publications with HP and Muscle
 Ovary Antibodies  >130 publications with HP and Ovary
 Testis Antibodies  >48 publications with HP and Testis
 Uncharacterized Tissue Antibodies  >1 publications with HP and Uncharacterized Tissue
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