Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow HIF-1alpha rightarrow Sheep HIF-1alpha  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • RBAK Antibody
 • ALDH1A2 Antibody
 • REQU Antibody
 • Voltage Dependent Anion Channel 1 (VDAC-1) ELISA Kit
 • Protein Kinase Inhibitor Gamma (PKIG) ELISA Kit
 • beta2-Glycoprotein 1 Antibody IgA (beta2-GP1 AB IgA) ELISA Kit
 • Alpha Synuclein (SNCA) ELISA Kit
 • Angiopoietin Like Protein 1 (ANGPTL1) ELISA Kit
 • Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit
 • Dehydroepiandrosterone (DHEA) ELISA Kit
 • Adenosine Triphosphate (ATP) ELISA Kit
 • Phospho-Nuclear Factor Kappa B (PNFKB) ELISA Kit
 • Nicotinic Acetylcholine Transferase Receptor (N-ACHTR) ELISA Kit
 • Tata Box Binding Protein Associated Factor 2 (TAF2) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

HIF-1alpha elisa kit :: Sheep Hypoxia Inducible Factor 1, Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS010181 HIF-1alpha elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hypoxia Inducible Factor 1, Alpha (HIF-1alpha), ELISA Kit

 Also Known As   

Sheep Hypoxia Inducible Factor 1, Alpha ELISA Kit

 Product Gene Name   

HIF-1alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 14; NC_000014.8 (62162119..62214977). Location: 14q23.2
 OMIM    603348
 3D Structure    ModBase 3D Structure for Q16665
Section Bar
 Species Reactivity    Sheep
Section Bar
Section Bar
 Assay Type    Sandwich
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF-1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF-1alpha purchase    MBS010181 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 1, Alpha (HIF-1alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HIF-1alpha. The ELISA analytical biochemical technique of the MBS010181 kit is based on HIF-1alpha antibody-HIF-1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF-1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF-1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for HIF-1alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4504385
 NCBI GeneID    3091
 NCBI Accession #    NP_001521.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001530.3 [Other Products]
Section Bar
 UniProt Primary Accession #    Q16665 [Other Products]
 UniProt Secondary Accession #    Q53XP6; Q96PT9; Q9UPB1; C0LZJ3 [Other Products]
 UniProt Related Accession #    Q16665 [Other Products]
 Molecular Weight    92,670 Da
Table BarTOPTable Bar
 NCBI Official Full Name    hypoxia-inducible factor 1-alpha isoform 1
 NCBI Official Synonym Full Names    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    HIF1A [Similar Products]
 NCBI Official Synonym Symbols   
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; hypoxia-inducible factor1alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 UniProt Synonym Protein Names   
ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8
 UniProt Gene Name    HIF1A [Similar Products]
 UniProt Synonym Gene Names    BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78 [Similar Products]
 UniProt Entry Name    HIF1A_HUMAN
Section Bar
 NCBI Summary for HIF-1alpha    This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Section Bar
 UniProt Comments for HIF-1alpha    HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone deacetylase binding; Hsp90 protein binding; protein kinase binding; transcription factor binding; histone acetyltransferase binding; signal transducer activity; protein binding; enzyme binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: lactation; oxygen homeostasis; embryonic placenta development; response to muscle activity; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; signal transduction; glucose homeostasis; positive regulation of vascular endothelial growth factor receptor signaling pathway; muscle maintenance; negative regulation of bone mineralization; connective tissue replacement during inflammatory response; elastin metabolic process; axon transport of mitochondrion; regulation of transcription, DNA-dependent; visual learning; heart looping; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; neural crest cell migration; negative regulation of growth; hemoglobin biosynthetic process; positive regulation of neuroblast proliferation; regulation of transforming growth factor-beta2 production; Notch signaling pathway; negative regulation of TOR signaling pathway; collagen metabolic process; embryonic hemopoiesis; positive regulation of nitric-oxide synthase activity; positive regulation of erythrocyte differentiation; B-1 B cell homeostasis; digestive tract morphogenesis; mRNA transcription from RNA polymerase II promoter; positive regulation of chemokine production; positive regulation of angiogenesis; neural fold elevation formation; regulation of gene expression; cartilage development; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; lactate metabolic process; response to hypoxia; epithelial to mesenchymal transition; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development
Table BarTOPTable Bar
 Research Articles on HIF-1alpha    1. Study links hTRM9L and tRNA modifications to inhibition of tumour growth via LIN9 and HIF1-alpha-dependent mechanisms.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with HIF-1alpha elisa kitPathways associated with HIF-1alpha elisa kit
 Reference Product  PubMed Publications
 VHL elisa kit  >469 publications with HIF-1alpha and VHL
 ARNT elisa kit  >265 publications with HIF-1alpha and ARNT
 EGLN1 elisa kit  >116 publications with HIF-1alpha and EGLN1
 EGLN3 elisa kit  >60 publications with HIF-1alpha and EGLN3
 HIF1AN elisa kit  >55 publications with HIF-1alpha and HIF1AN
 TP53 elisa kit  >29 publications with HIF-1alpha and TP53
 EP300 elisa kit  >21 publications with HIF-1alpha and EP300
 CREBBP elisa kit  >13 publications with HIF-1alpha and CREBBP
 UBC elisa kit  >4 publications with HIF-1alpha and UBC
 HSP90AA1 elisa kit  >3 publications with HIF-1alpha and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with HIF-1alpha elisa kitOrgans/Tissues associated with HIF-1alpha elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2859 publications with HIF-1alpha and Neoplasms
 Carcinoma Antibodies  >1194 publications with HIF-1alpha and Carcinoma
 Nervous System Diseases Antibodies  >574 publications with HIF-1alpha and Nervous System Diseases
 Inflammation Antibodies  >430 publications with HIF-1alpha and Inflammation
 Brain Diseases Antibodies  >420 publications with HIF-1alpha and Brain Diseases
 Breast Neoplasms Antibodies  >369 publications with HIF-1alpha and Breast Neoplasms
 Kidney Diseases Antibodies  >368 publications with HIF-1alpha and Kidney Diseases
 Lung Diseases Antibodies  >344 publications with HIF-1alpha and Lung Diseases
 Necrosis Antibodies  >321 publications with HIF-1alpha and Necrosis
 Liver Diseases Antibodies  >314 publications with HIF-1alpha and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >634 publications with HIF-1alpha and Brain
 Muscle Antibodies  >625 publications with HIF-1alpha and Muscle
 Kidney Antibodies  >594 publications with HIF-1alpha and Kidney
 Lung Antibodies  >519 publications with HIF-1alpha and Lung
 Liver Antibodies  >508 publications with HIF-1alpha and Liver
 Bone Antibodies  >356 publications with HIF-1alpha and Bone
 Prostate Antibodies  >249 publications with HIF-1alpha and Prostate
 Embryonic Tissue Antibodies  >247 publications with HIF-1alpha and Embryonic Tissue
 Bone Marrow Antibodies  >160 publications with HIF-1alpha and Bone Marrow
 Lymph Node Antibodies  >148 publications with HIF-1alpha and Lymph Node
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions