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ICAM-1 elisa kit :: Sheep Intercellular Adhesion Molecule 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735092
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Intercellular Adhesion Molecule 1 (ICAM-1), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Intercellular Adhesion Molecule 1 ELISA Kit

 Product Gene Name   

ICAM-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 611162
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 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ICAM-1. No significant cross-reactivity or interference between ICAM-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ICAM-1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This ICAM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ICAM-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ICAM-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ICAM-1 purchase    MBS735092 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intercellular Adhesion Molecule 1 (ICAM-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing ICAM-1, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS735092 kit is based on ICAM-1 antibody-ICAM-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ICAM-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ICAM-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for ICAM-1 elisa kit

   Principle of the assay: ICAM-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ICAM-1. Standards or samples are then added to the microtiter plate wells and ICAM-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ICAM-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ICAM-1 are added to each well to "sandwich" the ICAM-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ICAM-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICAM-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ICAM-1 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of ICAM-1 elisa kit    ICAM-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735092. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ICAM-1. It may not necessarily be applicable to this product.
 NCBI GI #    167466198
 NCBI GeneID    3383
 NCBI Accession #    NP_000192.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000201.2 [Other Products]
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 UniProt Secondary Accession #    Q5NKV7; Q96B50; B2R6M3 [Other Products]
 UniProt Related Accession #    P05362 [Other Products]
 Molecular Weight    57,825 Da
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 NCBI Official Full Name    intercellular adhesion molecule 1
 NCBI Official Synonym Full Names    intercellular adhesion molecule 1
 NCBI Official Symbol    ICAM1 [Similar Products]
 NCBI Official Synonym Symbols   
BB2; CD54; P3.58
[Similar Products]
 NCBI Protein Information    intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
 UniProt Protein Name    Intercellular adhesion molecule 1
 UniProt Synonym Protein Names   
Major group rhinovirus receptor; CD_antigen: CD54
 Protein Family    Intercellular adhesion molecule
 UniProt Gene Name    ICAM1 [Similar Products]
 UniProt Synonym Gene Names    ICAM-1 [Similar Products]
 UniProt Entry Name    ICAM1_HUMAN
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 NCBI Summary for ICAM-1    This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ICAM-1    ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: entry of virus into host cell; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To
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 Research Articles on ICAM-1    1. K469E gene polymorphism of ICAM-1 was related to acute coronary syndrome recurrence and cardiovascular mortality
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ICAM-1 elisa kitPathways associated with ICAM-1 elisa kit
 Reference Product  PubMed Publications
 ITGB2 elisa kit  >878 publications with ICAM-1 and ITGB2
 ITGAL elisa kit  >471 publications with ICAM-1 and ITGAL
 ITGAM elisa kit  >437 publications with ICAM-1 and ITGAM
 CD44 elisa kit  >367 publications with ICAM-1 and CD44
 IL6 elisa kit  >145 publications with ICAM-1 and IL6
 ITGAX elisa kit  >139 publications with ICAM-1 and ITGAX
 SELE elisa kit  >74 publications with ICAM-1 and SELE
 HLA-B elisa kit  >29 publications with ICAM-1 and HLA-B
 DIF elisa kit  >3 publications with ICAM-1 and DIF
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
Diseases associated with ICAM-1 elisa kitOrgans/Tissues associated with ICAM-1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5497 publications with ICAM-1 and Inflammation
 Necrosis Antibodies  >4714 publications with ICAM-1 and Necrosis
 Cardiovascular Diseases Antibodies  >3562 publications with ICAM-1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1425 publications with ICAM-1 and Atherosclerosis
 Reperfusion Injury Antibodies  >866 publications with ICAM-1 and Reperfusion Injury
 Myocardial Ischemia Antibodies  >786 publications with ICAM-1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >750 publications with ICAM-1 and Kidney Diseases
 Liver Diseases Antibodies  >543 publications with ICAM-1 and Liver Diseases
 Hypertension Antibodies  >514 publications with ICAM-1 and Hypertension
 Asthma Antibodies  >498 publications with ICAM-1 and Asthma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11619 publications with ICAM-1 and Blood
 Vascular Antibodies  >8687 publications with ICAM-1 and Vascular
 Lung Antibodies  >1742 publications with ICAM-1 and Lung
 Heart Antibodies  >1260 publications with ICAM-1 and Heart
 Skin Antibodies  >1191 publications with ICAM-1 and Skin
 Liver Antibodies  >1168 publications with ICAM-1 and Liver
 Kidney Antibodies  >1154 publications with ICAM-1 and Kidney
 Brain Antibodies  >1152 publications with ICAM-1 and Brain
 Muscle Antibodies  >1024 publications with ICAM-1 and Muscle
 Embryonic Tissue Antibodies  >770 publications with ICAM-1 and Embryonic Tissue
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