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gamma IFN-Ab elisa kit :: Sheep Interferon gamma antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS742190
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interferon gamma antibody (gamma IFN-Ab), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Interferon gamma antibody ELISA Kit

 Product Synonym Names    Sheep Interferon g antibody ELISA Kit
 Product Gene Name   

gamma IFN-Ab elisa kit

[Similar Products]
 Product Synonym Gene Name    g IFN-Ab [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IFN?Ab. No significant cross-reactivity or interference between IFN?Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFN?Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Sensitivity    0.1 ng/mL.
 Intended Uses    This IFN?Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IFN?Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of gamma IFN-Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for gamma IFN-Ab purchase    MBS742190 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon gamma antibody (gamma IFN-Ab) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing gamma IFN-Ab, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS742190 kit is based on gamma IFN-Ab antibody-gamma IFN-Ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect gamma IFN-Ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, gamma IFN-Ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for gamma IFN-Ab elisa kit

   Principle of the assay: IFN?Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with? Interferon antigen. Standards or samples are then added to the microtiter plate wells and IFN?Ab if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of IFN?Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated ? Interferon antigen is added to each well to “sandwich” the IFN?Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN?Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFN?Ab concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for gamma IFN-Ab elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of gamma IFN-Ab elisa kit    gamma IFN-Ab elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS742190. Click to request current manual
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NCBI/Uniprot data below describe general gene information for gamma IFN-Ab. It may not necessarily be applicable to this product.
 NCBI GI #    302393600
 NCBI GeneID    449517
 NCBI Accession #    NP_001180594.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001193665.1 [Other Products]
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 UniProt Related Accession #    Q9TTB0 [Other Products]
 Molecular Weight    16,804 Da
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 NCBI Official Full Name    interferon gamma
 NCBI Official Symbol    IFNG [Similar Products]
 NCBI Protein Information    interferon gamma; IFN-gamma; immune interferon
 UniProt Protein Name    Interferon gamma
 UniProt Synonym Protein Names   
Immune interferon
 UniProt Gene Name    IFNG [Similar Products]
 UniProt Synonym Gene Names    IFN-gamma [Similar Products]
 UniProt Entry Name    IFNG_PANTR
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 UniProt Comments for gamma IFN-Ab    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons ().
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 Research Articles on gamma IFN-Ab    1. CD3(-) CD8(+) NK cells play a vital role in controlling HIV-1 infection by producing high levels of IFN-gamma, and that IL-15 elicits IFN-gamma production in this subpopulation of NK cells in HIV-1-infected chimpanzees. [Il-15, CD8 antigen, IFN-gamma]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with gamma IFN-Ab elisa kitDiseases associated with gamma IFN-Ab elisa kit
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >139 publications with gamma IFN-Ab and Necrosis
 Autoimmune Diseases Antibodies  >84 publications with gamma IFN-Ab and Autoimmune Diseases
 Respiratory Tract Diseases Antibodies  >63 publications with gamma IFN-Ab and Respiratory Tract Diseases
 Hepatitis Antibodies  >31 publications with gamma IFN-Ab and Hepatitis
 Lymphoproliferative Disorders Antibodies  >20 publications with gamma IFN-Ab and Lymphoproliferative Disorders
 Diabetes Mellitus, Type 1 Antibodies  >17 publications with gamma IFN-Ab and Diabetes Mellitus, Type 1
 HIV Infections Antibodies  >16 publications with gamma IFN-Ab and HIV Infections
 Arthritis, Rheumatoid Antibodies  >16 publications with gamma IFN-Ab and Arthritis, Rheumatoid
 Hepatitis C Antibodies  >13 publications with gamma IFN-Ab and Hepatitis C
 Leishmaniasis Antibodies  >9 publications with gamma IFN-Ab and Leishmaniasis
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