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IL-12 elisa kit :: Sheep Interleukin 12, IL-12 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700980
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
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 Product Name   

Interleukin 12, IL-12, ELISA Kit

★Popular Item★
 Also Known As   

Sheep Interleukin 12, IL-12 ELISA Kit

 Product Synonym Names    Sheep Interleukin 12; IL-12 ELISA Kit; Interleukin 12; IL-12
 Product Gene Name   

IL-12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700980 Testing Data    Testing Data PDF
 OMIM    601642
 3D Structure    ModBase 3D Structure for Q99665
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 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of sheep IL-12. No significant cross-reactivity or interference between sheep IL-12 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml.
 Sensitivity    The minimum detectable dose of sheep IL-12 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-12 purchase    MBS700980 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 12, IL-12, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing IL-12, with an estimated sensitivity of The minimum detectable dose of sheep IL-12 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700980 kit is based on IL-12 antibody-IL-12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-12 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL-12 elisa kit    IL-12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700980. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-12. It may not necessarily be applicable to this product.
 NCBI GI #    1685028
 NCBI GeneID    3595
 NCBI Accession #    AAB36675.1 [Other Products]
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 UniProt Primary Accession #    Q99665 [Other Products]
 UniProt Secondary Accession #    Q2M3V3; B1AN98; B7ZKL9; F5H7L6 [Other Products]
 UniProt Related Accession #    Q99665 [Other Products]
 Molecular Weight    87,484 Da
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 NCBI Official Full Name    Il-12 receptor beta2
 NCBI Official Synonym Full Names    interleukin 12 receptor, beta 2
 NCBI Official Symbol    IL12RB2 [Similar Products]
 NCBI Protein Information    interleukin-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12 receptor subunit beta-2; interleukin-12 receptor beta-2 chain
 UniProt Protein Name    Interleukin-12 receptor subunit beta-2
 UniProt Gene Name    IL12RB2 [Similar Products]
 UniProt Synonym Gene Names    IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2 [Similar Products]
 UniProt Entry Name    I12R2_HUMAN
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 NCBI Summary for IL-12    The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]
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 UniProt Comments for IL-12    Function: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
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 Research Articles on IL-12    1. ERbeta2 and IL-12Rbeta5 had longer OS.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-12 elisa kitPathways associated with IL-12 elisa kit
 Reference Product  PubMed Publications
 IL12RB1 elisa kit  >50 publications with IL-12 and IL12RB1
 STAT4 elisa kit  >35 publications with IL-12 and STAT4
 IL12A elisa kit  >12 publications with IL-12 and IL12A
 IL12B elisa kit  >11 publications with IL-12 and IL12B
 TBX21 elisa kit  >10 publications with IL-12 and TBX21
 IFNG elisa kit  >6 publications with IL-12 and IFNG
 STAT1 elisa kit  >3 publications with IL-12 and STAT1
 TYK2 elisa kit  >2 publications with IL-12 and TYK2
 JAK2 elisa kit  >2 publications with IL-12 and JAK2
 SOCS3 elisa kit  >1 publications with IL-12 and SOCS3
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 IL27-mediated Signaling Events Pathway antibodies  IL27-mediated Signaling Events Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with IL-12 elisa kitOrgans/Tissues associated with IL-12 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >22 publications with IL-12 and Inflammation
 Nervous System Diseases Antibodies  >16 publications with IL-12 and Nervous System Diseases
 Lung Diseases Antibodies  >11 publications with IL-12 and Lung Diseases
 Necrosis Antibodies  >10 publications with IL-12 and Necrosis
 Liver Diseases Antibodies  >9 publications with IL-12 and Liver Diseases
 Brain Diseases Antibodies  >9 publications with IL-12 and Brain Diseases
 Asthma Antibodies  >7 publications with IL-12 and Asthma
 Cardiovascular Diseases Antibodies  >6 publications with IL-12 and Cardiovascular Diseases
 Intestinal Diseases Antibodies  >6 publications with IL-12 and Intestinal Diseases
 Lung Neoplasms Antibodies  >5 publications with IL-12 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >103 publications with IL-12 and Blood
 Liver Antibodies  >8 publications with IL-12 and Liver
 Skin Antibodies  >7 publications with IL-12 and Skin
 Thymus Antibodies  >3 publications with IL-12 and Thymus
 Brain Antibodies  >2 publications with IL-12 and Brain
 Mixed Antibodies  >1 publications with IL-12 and Mixed
 Embryonic Tissue Antibodies  >1 publications with IL-12 and Embryonic Tissue
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