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Leptin elisa kit :: Sheep Leptin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS742106
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin, ELISA Kit

★Popular Item★
 Also Known As   

Sheep Leptin ELISA Kit

 Product Gene Name   

Leptin elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS742106 COA    COA PDF
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Leptin. No significant cross-reactivity or interference between Leptin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Leptin and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Leptin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Leptin purchase    MBS742106 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leptin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Leptin, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS742106 kit is based on Leptin antibody-Leptin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Leptin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Leptin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Leptin elisa kit

   Intended Uses: This Leptin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Leptin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Leptin ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Leptin antibody and an Leptin-HRP conjugate. The assay sample and buffer are incubated together with Leptin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin concentration since Leptin from samples and Leptin-HRP conjugate compete for the anti-Leptin antibody binding site. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Leptin elisa kit    Signal Transduction
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  Typical Testing Data/Standard Curve (for reference only) of Leptin elisa kit    Leptin elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS742106. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Leptin. It may not necessarily be applicable to this product.
 NCBI GI #    148233267
 NCBI GeneID    734226
 NCBI Accession #    NP_001089183.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001095714.1 [Other Products]
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 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin<
 NCBI Official Symbol    lep [Similar Products]
 NCBI Official Synonym Symbols   
ob; xlep
[Similar Products]
 NCBI Protein Information    leptin; obesity homolog
 Protein Family    Leptin
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 Research Articles on Leptin    1. Leptin can influence limb growth and differentiation during early development. (leptin)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Leptin elisa kit
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
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