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M-AChRM2 elisa kit :: Sheep M-AChR M2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2507960
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $585 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

M-AChR M2 (M-AChRM2), ELISA Kit

 Also Known As   

Sheep M-AChR M2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

 Product Gene Name   

M-AChRM2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9ERZ4
 Species Reactivity    Sheep
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 Specificity    This kit recognizes natural and recombinant Sheep M-AChRM2. No significant cross-reactivity or interference between Sheep M-AChRM2 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of M-AChRM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for M-AChRM2 purchase    MBS2507960 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the M-AChR M2 (M-AChRM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing M-AChRM2, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2507960 kit is based on M-AChRM2 antibody-M-AChRM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect M-AChRM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, M-AChRM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for M-AChRM2 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep M-AChRM2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to M-AChRM2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for M-AChRM2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain M-AChRM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of M-AChRM2. You can calculate the concentration of M-AChRM2 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of M-AChRM2 elisa kit    M-AChRM2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2507960. Click to request current manual
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NCBI/Uniprot data below describe general gene information for M-AChRM2. It may not necessarily be applicable to this product.
 NCBI GI #    282721106
 NCBI GeneID    243764
 NCBI Accession #    NP_987076.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_203491.3 [Other Products]
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 UniProt Primary Accession #    Q9ERZ4 [Other Products]
 UniProt Secondary Accession #    E9Q9P0 [Other Products]
 UniProt Related Accession #    Q9ERZ4 [Other Products]
 Molecular Weight    51,499 Da
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 NCBI Official Full Name    muscarinic acetylcholine receptor M2
 NCBI Official Synonym Full Names    cholinergic receptor, muscarinic 2, cardiac
 NCBI Official Symbol    Chrm2 [Similar Products]
 NCBI Official Synonym Symbols   
M2; Chrm-2; AChR-M2
[Similar Products]
 NCBI Protein Information    muscarinic acetylcholine receptor M2; AChR M2; muscarinic acetylcholine receptor 2
 UniProt Protein Name    Muscarinic acetylcholine receptor M2
 UniProt Gene Name    Chrm2 [Similar Products]
 UniProt Synonym Gene Names    Chrm-2 [Similar Products]
 UniProt Entry Name    ACM2_MOUSE
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 UniProt Comments for M-AChRM2    mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Cellular Component: postsynaptic membrane; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction; cytosol

Molecular Function: G-protein coupled receptor activity; signal transducer activity; G-protein coupled acetylcholine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); acetylcholine receptor signaling, muscarinic pathway; signal transduction; regulation of heart contraction
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 Research Articles on M-AChRM2    1. Beta-2-Adrenergic and M2-muscarinic receptors decrease basal t-tubular L-type calcium channel activity and suppress ventricular contractility in heart failure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with M-AChRM2 elisa kitDiseases associated with M-AChRM2 elisa kit
 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Substance-Related Disorders Antibodies  >24 publications with M-AChRM2 and Substance-Related Disorders
 Nervous System Diseases Antibodies  >4 publications with M-AChRM2 and Nervous System Diseases
 Inflammation Antibodies  >4 publications with M-AChRM2 and Inflammation
 Lung Diseases Antibodies  >4 publications with M-AChRM2 and Lung Diseases
 Death Antibodies  >4 publications with M-AChRM2 and Death
 Cognition Disorders Antibodies  >3 publications with M-AChRM2 and Cognition Disorders
 Memory Disorders Antibodies  >3 publications with M-AChRM2 and Memory Disorders
 Immune System Diseases Antibodies  >3 publications with M-AChRM2 and Immune System Diseases
 Weight Loss Antibodies  >1 publications with M-AChRM2 and Weight Loss
 Poisoning Antibodies  >1 publications with M-AChRM2 and Poisoning
Organs/Tissues associated with M-AChRM2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with M-AChRM2 and Brain
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