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NEFA elisa kit :: Sheep Non-ester Fatty Acid ELISA Kit

Scan QR to view Datasheet Catalog #    MBS751414
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Non-ester Fatty Acid (NEFA), ELISA Kit

 Also Known As   

Sheep Non-ester Fatty Acid ELISA Kit

 Product Gene Name   

NEFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Sheep
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Intended Uses    This BG NEFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep NEFA. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intended Uses    This BG NEFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep NEFA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of NEFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NEFA purchase    MBS751414 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Non-ester Fatty Acid (NEFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEFA. The ELISA analytical biochemical technique of the MBS751414 kit is based on NEFA antibody-NEFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for NEFA elisa kit

   Principle of the Assay: NEFA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NEFA antibody and an NEFA-HRP conjugate. The assay sample and buffer are incubated together with NEFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFA concentration since NEFA from samples and NEFA-HRP conjugate compete for the anti-NEFA antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFA from the sample, fewer sites are left to bind NEFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFA concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NEFA elisa kit    NEFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS751414
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NCBI/Uniprot data below describe general gene information for NEFA. It may not necessarily be applicable to this product.
 NCBI GI #    8250669
 NCBI Accession #    AAA96709.2 [Other Products]
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 UniProt Secondary Accession #    Q67213 [Other Products]
 Molecular Weight    51,888 Da
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 NCBI Official Full Name    neuraminidase
 UniProt Protein Name    Neuraminidase
 UniProt Gene Name    NA [Similar Products]
 UniProt Entry Name    NRAM_I57A1
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 UniProt Comments for NEFA    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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