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PGAM2 elisa kit :: Sheep PGAM2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515614 PGAM2 elisa kit
Unit / Price
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $585 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,475 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,360 +5 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


 Also Known As   

Sheep PGAM2 (Phosphoglycerate Mutase 2) ELISA Kit

 Product Gene Name   

PGAM2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    261670
 3D Structure    ModBase 3D Structure for P15259
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 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Sheep PGAM2. No significant cross-reactivity or interference between Sheep PGAM2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Sheep PGAM2 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PGAM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PGAM2 purchase    MBS2515614 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PGAM2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PGAM2. The ELISA analytical biochemical technique of the MBS2515614 kit is based on PGAM2 antibody-PGAM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGAM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGAM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PGAM2 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PGAM2 in Sheep serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sheep PGAM2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Sheep PGAM2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheep PGAM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheep PGAM2. You can calculate the concentration of Sheep PGAM2 in the samples by comparing the OD of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for PGAM2. It may not necessarily be applicable to this product.
 NCBI GI #    50593010
 NCBI GeneID    5224
 NCBI Accession #    NP_000281.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000290.3 [Other Products]
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 UniProt Primary Accession #    P15259 [Other Products]
 UniProt Related Accession #    P15259 [Other Products]
 Molecular Weight    28,766 Da
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 NCBI Official Full Name    phosphoglycerate mutase 2
 NCBI Official Synonym Full Names    phosphoglycerate mutase 2 (muscle)
 NCBI Official Symbol    PGAM2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    phosphoglycerate mutase 2; BPG-dependent PGAM 2; muscle-specific phosphoglycerate mutase; phosphoglycerate mutase isozyme M
 UniProt Protein Name    Phosphoglycerate mutase 2
 UniProt Synonym Protein Names   
BPG-dependent PGAM 2; Muscle-specific phosphoglycerate mutase; Phosphoglycerate mutase isozyme M; PGAM-M
 Protein Family    Phosphoglycerate mutase
 UniProt Gene Name    PGAM2 [Similar Products]
 UniProt Synonym Gene Names    PGAMM; PGAM-M [Similar Products]
 UniProt Entry Name    PGAM2_HUMAN
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 NCBI Summary for PGAM2    Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]
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 UniProt Comments for PGAM2    PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC (synthase) and EC (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC; Phosphatase (non-protein); EC; EC; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 7p13-p12

Cellular Component: nucleus; cytosol

Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity

Biological Process: response to mercury ion; striated muscle contraction; glycolysis; dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; spermatogenesis; gluconeogenesis

Disease: Glycogen Storage Disease X
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 Research Articles on PGAM2    1. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PGAM2 elisa kitPathways associated with PGAM2 elisa kit
 Reference Product  PubMed Publications
 GPI elisa kit  >3 publications with PGAM2 and GPI
 ALDOA elisa kit  >1 publications with PGAM2 and ALDOA
 BPGM elisa kit  >1 publications with PGAM2 and BPGM
 ENO1 elisa kit  >1 publications with PGAM2 and ENO1
 PGK1 elisa kit  >1 publications with PGAM2 and PGK1
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Diseases associated with PGAM2 elisa kitOrgans/Tissues associated with PGAM2 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3 publications with PGAM2 and Necrosis
 Fibrosis Antibodies  >2 publications with PGAM2 and Fibrosis
 Abnormalities, Multiple Antibodies  >1 publications with PGAM2 and Abnormalities, Multiple
 Heart Failure Antibodies  >1 publications with PGAM2 and Heart Failure
 Weight Gain Antibodies  >1 publications with PGAM2 and Weight Gain
 Nervous System Diseases Antibodies  >1 publications with PGAM2 and Nervous System Diseases
 Death Antibodies  >1 publications with PGAM2 and Death
 Liver Diseases Antibodies  >1 publications with PGAM2 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with PGAM2 and Kidney Diseases
 Dimauro disease Antibodies  >1 publications with PGAM2 and Dimauro disease
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >14 publications with PGAM2 and Muscle
 Heart Antibodies  >3 publications with PGAM2 and Heart
 Lung Antibodies  >3 publications with PGAM2 and Lung
 Embryonic Tissue Antibodies  >2 publications with PGAM2 and Embryonic Tissue
 Bone Antibodies  >1 publications with PGAM2 and Bone
 Brain Antibodies  >1 publications with PGAM2 and Brain
 Testis Antibodies  >1 publications with PGAM2 and Testis
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