Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PECAM-1/CD31 rightarrow Sheep PECAM-1/CD31  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Indoleamine-2,3-Dioxygenase (IDO) ELISA Kit
 • N (2)-fixation sustaining protein CowN (AZC_4276) Recombinant Protein
 • ADSL Antibody
 • F4-80 Antibody
 • Tetracycline Antibody
 • Arrestin beta 2 (ARRbeta2) ELISA Kit
 • Neuropeptide Y (NP-Y) ELISA Kit
 • GERP-TRIM8 Antibody
 • RARG Antibody
 • Heparan Sulphate Proteoglycan (HSPG) ELISA Kit
 • VPS28 Recombinant Protein
 • Myoglobin (MB) Protein
 • G-rich RNA sequence binding factor 1 (GRSF1) ELISA Kit
 • RNA 5.8s, Ribosomal Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PECAM-1/CD31 elisa kit :: Sheep Platelet endothelial cell adhesion molecule-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740187
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), ELISA Kit

 Also Known As   

Sheep Platelet endothelial cell adhesion molecule-1 ELISA Kit

 Product Gene Name   

PECAM-1/CD31 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    173445
Section Bar
 Species Reactivity    Sheep
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This PECAM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PECAM-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PECAM-1/CD31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PECAM-1/CD31 purchase    MBS740187 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PECAM-1/CD31, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS740187 kit is based on PECAM-1/CD31 antibody-PECAM-1/CD31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PECAM-1/CD31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PECAM-1/CD31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for PECAM-1/CD31 elisa kit

   Principle of the Assay: PECAM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PECAM-1 antibody and an PECAM-1-HRP conjugate. The assay sample and buffer are incubated together with PECAM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PECAM-1 concentration since PECAM-1 from samples and PECAM-1-HRP conjugate compete for the anti-PECAM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PECAM-1 from the sample, fewer sites are left to bind PECAM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PECAM-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for PECAM-1/CD31 elisa kit    Cardiovascular
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PECAM-1/CD31 elisa kit    PECAM-1/CD31 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS740187
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PECAM-1/CD31. It may not necessarily be applicable to this product.
 NCBI GI #    313760624
 NCBI GeneID    5175
 NCBI Accession #    NP_000433.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_000442.4 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; Q9NQS9; Q9NQT0; A8K3S7; D3DU31 [Other Products]
 UniProt Related Accession #    P16284 [Other Products]
 Molecular Weight    81,378 Da
Table BarTOPTable Bar
 NCBI Official Full Name    platelet endothelial cell adhesion molecule
 NCBI Official Synonym Full Names    platelet/endothelial cell adhesion molecule 1
 NCBI Official Symbol    PECAM1 [Similar Products]
 NCBI Official Synonym Symbols   
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
[Similar Products]
 NCBI Protein Information    platelet endothelial cell adhesion molecule; CD31 antigen
 UniProt Protein Name    Platelet endothelial cell adhesion molecule
 UniProt Synonym Protein Names   
EndoCAM; GPIIA'; PECA1; CD_antigen: CD31
 UniProt Gene Name    PECAM1 [Similar Products]
 UniProt Synonym Gene Names    PECAM-1 [Similar Products]
 UniProt Entry Name    PECA1_HUMAN
Section Bar
 NCBI Summary for PECAM-1/CD31    The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Section Bar
 UniProt Comments for PECAM-1/CD31    PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; cell adhesion; signal transduction; blood coagulation; leukocyte migration; phagocytosis
Table BarTOPTable Bar
 Research Articles on PECAM-1/CD31    1. results of the current meta-analysis suggest that homozygous polymorphic genotype (GG) of the rs1131012 SNP may confer protection against myocardial infarction
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with PECAM-1/CD31 elisa kitDiseases associated with PECAM-1/CD31 elisa kit
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >616 publications with PECAM-1/CD31 and Cardiovascular Diseases
 Inflammation Antibodies  >486 publications with PECAM-1/CD31 and Inflammation
 Necrosis Antibodies  >399 publications with PECAM-1/CD31 and Necrosis
 Neoplasms, Experimental Antibodies  >258 publications with PECAM-1/CD31 and Neoplasms, Experimental
 Lung Diseases Antibodies  >234 publications with PECAM-1/CD31 and Lung Diseases
 Heart Diseases Antibodies  >229 publications with PECAM-1/CD31 and Heart Diseases
 Breast Neoplasms Antibodies  >204 publications with PECAM-1/CD31 and Breast Neoplasms
 Lung Neoplasms Antibodies  >183 publications with PECAM-1/CD31 and Lung Neoplasms
 Liver Diseases Antibodies  >171 publications with PECAM-1/CD31 and Liver Diseases
 Atherosclerosis Antibodies  >144 publications with PECAM-1/CD31 and Atherosclerosis
Organs/Tissues associated with PECAM-1/CD31 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3355 publications with PECAM-1/CD31 and Vascular
 Blood Antibodies  >2961 publications with PECAM-1/CD31 and Blood
 Muscle Antibodies  >677 publications with PECAM-1/CD31 and Muscle
 Embryonic Tissue Antibodies  >502 publications with PECAM-1/CD31 and Embryonic Tissue
 Lung Antibodies  >422 publications with PECAM-1/CD31 and Lung
 Heart Antibodies  >344 publications with PECAM-1/CD31 and Heart
 Skin Antibodies  >334 publications with PECAM-1/CD31 and Skin
 Brain Antibodies  >317 publications with PECAM-1/CD31 and Brain
 Connective Tissue Antibodies  >238 publications with PECAM-1/CD31 and Connective Tissue
 Kidney Antibodies  >231 publications with PECAM-1/CD31 and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions