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BGN elisa kit :: Sheep biglycan ELISA Kit

Scan QR to view Datasheet Catalog #    MBS941464
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,735 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,135 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

biglycan (BGN), ELISA Kit

 Also Known As   

Sheep Biglycan, BGN ELISA Kit

 Product Synonym Names    Sheep Biglycan (BGN) ELISA kit; DSPG1; PG-S1; PGI; SLRR1A; biglycan proteoglycan; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; small leucine-rich protein 1A; biglycan
 Product Gene Name   

BGN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS941464 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O46390
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of sheep BGN. No significant cross-reactivity or interference between sheep BGN and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.78 ng/ml -50 ng/ml.
 Sensitivity    The minimum detectable dose of sheep BGN is typically less than 0.19 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of BGN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BGN purchase    MBS941464 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the biglycan (BGN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range of 0.78 ng/ml -50 ng/ml in biological research samples containing BGN, with an estimated sensitivity of The minimum detectable dose of sheep BGN is typically less than 0.19 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS941464 kit is based on BGN antibody-BGN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BGN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BGN. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BGN elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of BGN elisa kit    BGN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS941464
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NCBI/Uniprot data below describe general gene information for BGN. It may not necessarily be applicable to this product.
 NCBI GI #    57163947
 NCBI GeneID    443011
 NCBI Accession #    NP_001009201.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001009201.1 [Other Products]
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 UniProt Primary Accession #    O46390 [Other Products]
 UniProt Related Accession #    O46390 [Other Products]
 Molecular Weight    41,523 Da
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 NCBI Official Full Name    biglycan
 NCBI Official Symbol    BGN [Similar Products]
 NCBI Protein Information    biglycan; PG-S1; bone/cartilage proteoglycan I
 UniProt Protein Name    Biglycan
 UniProt Synonym Protein Names   
Bone/cartilage proteoglycan I; PG-S1
 Protein Family    Biglycan
 UniProt Gene Name    BGN [Similar Products]
 UniProt Entry Name    PGS1_SHEEP
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 UniProt Comments for BGN    Function: May be involved in collagen fiber assembly
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with BGN elisa kitOrgans/Tissues associated with BGN elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >41 publications with BGN and Neoplasms
 Fibrosis Antibodies  >31 publications with BGN and Fibrosis
 Atherosclerosis Antibodies  >25 publications with BGN and Atherosclerosis
 Inflammation Antibodies  >23 publications with BGN and Inflammation
 Necrosis Antibodies  >20 publications with BGN and Necrosis
 Kidney Diseases Antibodies  >20 publications with BGN and Kidney Diseases
 Heart Diseases Antibodies  >18 publications with BGN and Heart Diseases
 Skin Diseases Antibodies  >16 publications with BGN and Skin Diseases
 Carcinoma Antibodies  >9 publications with BGN and Carcinoma
 Brain Diseases Antibodies  >8 publications with BGN and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >120 publications with BGN and Bone
 Muscle Antibodies  >75 publications with BGN and Muscle
 Skin Antibodies  >38 publications with BGN and Skin
 Digestive Antibodies  >33 publications with BGN and Digestive
 Female Genital Antibodies  >12 publications with BGN and Female Genital
 Hindlimb Antibodies  >3 publications with BGN and Hindlimb
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