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IGF1 elisa kit :: Sheep insulin-like growth factor 1 (somatomedin C) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700234
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

insulin-like growth factor 1 (somatomedin C) (IGF1), ELISA Kit

 Also Known As   

Sheep insulin-like growth factor 1, IGF-1 ELISA Kit

 Product Synonym Names    Sheep insulin-like growth factor 1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)
 Product Gene Name   

IGF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700234 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P10763
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of sheep IGF-1. No significant cross-reactivity or interference between sheep IGF-1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 ng/ml-1000 ng/ml.
 Sensitivity    The minimum detectable dose of sheep IGF-1 is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IGF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IGF1 purchase    MBS700234 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the insulin-like growth factor 1 (somatomedin C) (IGF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 ng/ml-1000 ng/ml in biological research samples containing IGF1, with an estimated sensitivity of The minimum detectable dose of sheep IGF-1 is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700234 kit is based on IGF1 antibody-IGF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IGF1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IGF1 elisa kit    IGF1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700234. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IGF1. It may not necessarily be applicable to this product.
 NCBI GI #    57526353
 NCBI GeneID    443318
 NCBI Accession #    NP_001009774.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001009774.3 [Other Products]
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 UniProt Primary Accession #    P10763 [Other Products]
 UniProt Related Accession #    P10763 [Other Products]
 Molecular Weight    17,012 Da
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 NCBI Official Full Name    insulin-like growth factor I
 NCBI Official Symbol    IGF1 [Similar Products]
 NCBI Official Synonym Symbols   
IGF-I
[Similar Products]
 NCBI Protein Information    insulin-like growth factor I; somatomedin
 UniProt Protein Name    Insulin-like growth factor I
 UniProt Synonym Protein Names   
Somatomedin
 Protein Family    Insulin-like growth factor
 UniProt Gene Name    IGF1 [Similar Products]
 UniProt Synonym Gene Names    IGF-I [Similar Products]
 UniProt Entry Name    IGF1_SHEEP
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 UniProt Comments for IGF1    Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
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 Research Articles on IGF1    1. Data show that both short- and long-term exposure to high-altitude conditions decreased luteal growth and IGF-I and IGF-II gene expression but increased HIF-1 alpha and VEGF immunoexpression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IGF1 elisa kitPathways associated with IGF1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with IGF1 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Diseases associated with IGF1 elisa kitOrgans/Tissues associated with IGF1 elisa kit
 Disease Name  Pubmed Publications
 Acromegaly Antibodies  >115 publications with IGF1 and Acromegaly
 Breast Neoplasms Antibodies  >106 publications with IGF1 and Breast Neoplasms
 Female Urogenital Diseases Antibodies  >80 publications with IGF1 and Female Urogenital Diseases
 Inflammation Antibodies  >73 publications with IGF1 and Inflammation
 Liver Diseases Antibodies  >56 publications with IGF1 and Liver Diseases
 Adenoma Antibodies  >53 publications with IGF1 and Adenoma
 Prostatic Neoplasms Antibodies  >45 publications with IGF1 and Prostatic Neoplasms
 Necrosis Antibodies  >44 publications with IGF1 and Necrosis
 Pituitary Neoplasms Antibodies  >44 publications with IGF1 and Pituitary Neoplasms
 Hypertension Antibodies  >36 publications with IGF1 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with IGF1 and Bone
 Connective Tissue Antibodies  >4 publications with IGF1 and Connective Tissue
 Heart Antibodies  >3 publications with IGF1 and Heart
 Muscle Antibodies  >3 publications with IGF1 and Muscle
 Brain Antibodies  >3 publications with IGF1 and Brain
 Vascular Antibodies  >2 publications with IGF1 and Vascular
 Embryonic Tissue Antibodies  >2 publications with IGF1 and Embryonic Tissue
 Ear Antibodies  >1 publications with IGF1 and Ear
 Kidney Antibodies  >1 publications with IGF1 and Kidney
 Umbilical Cord Antibodies  >1 publications with IGF1 and Umbilical Cord
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