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IL4 elisa kit :: Sheep interleukin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705670
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interleukin 4 (IL4), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Interleukin 4, IL-4 ELISA Kit

 Product Synonym Names    Sheep Interleukin 4 (IL-4) ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4
 Product Gene Name   

IL4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705670 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P30368
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of sheep IL-4. No significant cross-reactivity or interference between sheep IL-4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml -400 pg/ml
 Sensitivity    Typically less than 1.56 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL4 purchase    MBS705670 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 4 (IL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml -400 pg/ml in biological research samples containing IL4, with an estimated sensitivity of Typically less than 1.56 pg/ml. The ELISA analytical biochemical technique of the MBS705670 kit is based on IL4 antibody-IL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Interleukin 4 IL 4 has been identified as a crucial cytokine in the regulation of adaptive immunity. As a stimulatory factor for the differentiation of B cells to plasma antibody producing cells, this protein protects species against infectious diseases. Other functions and cellular regulation are being studied using one of MyBiosource's human IL 4 mouse IL 4 or sheep IL 4 IL4 ELISA kits. This IL 4 ELISA kit, like all kits interleukin family and receptor family, in our catalog, are validated for serum, tissue homogenates and cell culture supernates analysis. Interleukin 4 IL4 assay is designed as a ELISA kit sandwich format for pg ml detection using our plates pre coated with antibodies and standard curve calculations. Your results are easily comparable to other authors because all our cell growth factor and stimulatory factor 1 custom products provide highly reproducible data. View our immunol section of products at MyBiosource.com.
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Related Product Information for IL4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of IL4 elisa kit    IL4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705670. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL4. It may not necessarily be applicable to this product.
 NCBI GI #    57527820
 NCBI GeneID    443327
 NCBI Accession #    NP_001009313.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001009313.2 [Other Products]
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 UniProt Primary Accession #    P30368 [Other Products]
 UniProt Secondary Accession #    Q95MZ6 [Other Products]
 UniProt Related Accession #    P30368 [Other Products]
 Molecular Weight    15,136 Da
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 NCBI Official Full Name    interleukin-4
 NCBI Official Symbol    IL4 [Similar Products]
 NCBI Official Synonym Symbols   
IL-4
[Similar Products]
 NCBI Protein Information    interleukin-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
 UniProt Protein Name    Interleukin-4
 UniProt Synonym Protein Names   
B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
 Protein Family    Interleukin
 UniProt Gene Name    IL4 [Similar Products]
 UniProt Synonym Gene Names    IL-4; IL-4; BSF-1 [Similar Products]
 UniProt Entry Name    IL4_SHEEP
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 UniProt Comments for IL4    Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
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 Research Articles on IL4    1. Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL4 elisa kitDiseases associated with IL4 elisa kit
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >322 publications with IL4 and Inflammation
 Necrosis Antibodies  >318 publications with IL4 and Necrosis
 Hypersensitivity Antibodies  >314 publications with IL4 and Hypersensitivity
 Hypersensitivity, Immediate Antibodies  >252 publications with IL4 and Hypersensitivity, Immediate
 Asthma Antibodies  >219 publications with IL4 and Asthma
 Lung Diseases Antibodies  >204 publications with IL4 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >188 publications with IL4 and Respiratory Hypersensitivity
 Autoimmune Diseases Antibodies  >169 publications with IL4 and Autoimmune Diseases
 Liver Diseases Antibodies  >56 publications with IL4 and Liver Diseases
 Rhinitis Antibodies  >45 publications with IL4 and Rhinitis
Organs/Tissues associated with IL4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Digestive Antibodies  >101 publications with IL4 and Digestive
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