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IL6 elisa kit :: Sheep interleukin 6 (interferon, beta 2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS701895
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

interleukin 6 (interferon, beta 2) (IL6), ELISA Kit

★Popular Item★
 Also Known As   

Sheep Interleukin 6, IL-6 ELISA Kit

 Product Synonym Names    Sheep Interleukin 6; IL-6 ELISA Kit; BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2; B-cell differentiation factor; CTL differentiation factor; OTTHUMP00000158544; hybridoma growth factor; interleukin 6; interleukin BSF-2; interleukin 6 (interferon; beta 2)
 Product Gene Name   

IL6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS701895 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P29455
 Species Reactivity    Sheep
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Sheep IL6. No significant cross-reactivity or interference between Sheep IL6 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Competitive
 Detection Range    3.1 pg/ml-80 pg/ml
 Sensitivity    3.1 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 3    Acute phase
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL6 purchase    MBS701895 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interleukin 6 (interferon, beta 2) (IL6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.1 pg/ml-80 pg/ml in biological research samples containing IL6, with an estimated sensitivity of 3.1 pg/ml. The ELISA analytical biochemical technique of the MBS701895 kit is based on IL6 antibody-IL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL6 elisa kit

   Introduction: Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. In terms of host response to a foreign pathogen, IL-6 has been shown, in mice, to be required for resistance against the bacterium, Streptococcus pneumoniae. IL-6 is also a "myokine," a cytokine produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. During exercise, it is thought to act in a hormone-like manner to mobilize extracellular substrates and/or augment substrate delivery. Additionally, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. IL-6 is one of the most important mediators of fever and of the acute phase response. In the muscle and fatty tissue IL-6 stimulates energy mobilization which leads to increased body temperature. IL-6 can be secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPs). These PAMPs bind to highly important detection molecules of the innate immune system, called Toll-like receptors (TLRs), that are present on the cell surface (or in intracellular compartments) which induce intracellular signaling cascades that give rise to inflammatory cytokine production. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 (including estrogen) are used to treat postmenopausal osteoporosis.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-6 and Horseradish Peroxidase (HRP) conjugated IL-6, then incubated. Then substrate solutions are added to the wells, respectively. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IL6 elisa kit    IL6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS701895. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL6. It may not necessarily be applicable to this product.
 NCBI GI #    57164389
 NCBI GeneID    443406
 NCBI Accession #    NP_001009392.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001009392.1 [Other Products]
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 UniProt Primary Accession #    P29455 [Other Products]
 UniProt Related Accession #    P29455 [Other Products]
 Molecular Weight    23,446 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Symbol    IL6 [Similar Products]
 NCBI Protein Information    interleukin-6; IL-6
 UniProt Protein Name    Interleukin-6
 Protein Family    Interleukin
 UniProt Gene Name    IL6 [Similar Products]
 UniProt Synonym Gene Names    IL-6 [Similar Products]
 UniProt Entry Name    IL6_SHEEP
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 UniProt Comments for IL6    Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation
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 Research Articles on IL6    1. This is first report of single nucleotide polymorphism of IL-6 gene of Pakistani sheep.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL6 elisa kitDiseases associated with IL6 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2162 publications with IL6 and Inflammation
 Necrosis Antibodies  >1575 publications with IL6 and Necrosis
 Autoimmune Diseases Antibodies  >412 publications with IL6 and Autoimmune Diseases
 Skin Diseases Antibodies  >269 publications with IL6 and Skin Diseases
 Arthritis, Rheumatoid Antibodies  >250 publications with IL6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >222 publications with IL6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >216 publications with IL6 and Myocardial Ischemia
 Ischemia Antibodies  >113 publications with IL6 and Ischemia
 Myocardial Infarction Antibodies  >98 publications with IL6 and Myocardial Infarction
 Hypersensitivity Antibodies  >97 publications with IL6 and Hypersensitivity
Organs/Tissues associated with IL6 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with IL6 and Uncharacterized Tissue
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