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BH4 elisa kit :: Tetrahydrobiopterin (BH4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733839
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
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 Product Name   

Tetrahydrobiopterin (BH4), ELISA Kit

 Also Known As   

Human tetrahydrobiopterin (BH4) ELISA Kit

 Product Gene Name   

BH4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    PDF Manual
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 Specificity    This assay has high sensitivity and excellent specificity for detection of BH4. No significant cross-reactivity or interference between BH4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BH4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of BH4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BH4 purchase    MBS733839 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tetrahydrobiopterin (BH4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing BH4, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS733839 kit is based on BH4 antibody-BH4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BH4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BH4. Appropriate sample types include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BH4 elisa kit

   Intended Uses: This BH4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BH4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||BH4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BH4 antibody and an BH4-HRP conjugate. The assay sample and buffer are incubated together with BH4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BH4 concentration since BH4 from samples and BH4-HRP conjugate compete for the anti-BH4 antibody binding site. Since the number of sites is limited, as more sites are occupied by BH4 from the sample, fewer sites are left to bind BH4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BH4 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of BH4 elisa kit    BH4 elisa kit Typical Testing Data/Standard Curve image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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