Specificity
This kit exhibits no significant detectable cross-reactivity with HCG, hLH, hFSH, hProlactin, and hGH. Interference by HCG, hLH, hFSH, hProlactin, and hGH were measured by adding physiological amounts of each hormone into each blood sample.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our
technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Other Notes
Small volumes of TSH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TSH purchase
MBS590035 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyroid Stimulating Hormone (TSH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TSH. The ELISA analytical biochemical technique of the MBS590035 kit is based on TSH antibody-TSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TSH elisa kit
Principle of the assay: The Neonatal TSH quantitative enzyme immunoassay described as a solid phase enzyme linked immunosorbent assay (ELISA). Monoclonal antibodies, specific to TSH, have been bound to the surface of each microplate well. During the course of the assay, a blood sample (collected on filter paper) is added to the microplate wells with Sample Buffer and incubated overnight. After washing the microplate to remove the filter paper and unbound component of the sample, a standardized preparation of horseradish peroxidase-conjugated monoclonal antibody specific for TSH beta unit is added to each well and incubated. The TSH, if present in the sample, will bind to the antibody on the coated well and will form an Antibody-TSH-Antibody-HRP "sandwich". The microplate wells are thoroughly washed to remove unbound conjugate. Next, a TMB (3,3', 5,5' tetramthyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a 15-minute incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in colour. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of TSH in the test sample, this neonatal TSH ELISA Kit includes calibration standards and controls. The calibration standards and controls are assayed at the same time as the test samples and allow for the operator to produce a standard curve of optical density versus TSH muIU/mL, serum. Therefore, by comparing the optical density of the samples to this standard curve, the concentration of the TSH in the test samples is then determined.
Background: Thyroid-stimulating hormone (TSH) is secreted by the anterior lobe of the pituitary gland and induces the production and release thyroid hormones thyroxin (T4) and triiodothryronine (T3). These thyroid hormones exert a negative feedback on the pituitary. The release of TSH is regulated by TSH-releasing hormone (TRH) produced in the hypothalamus. When there are high circulating levels of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The normal concentration of TSH in the blood is extremely low, but it is essential for maintenance of normal thyroid function. The determination of serum or plasma levels of TSH is recognised as a sensitive method in the identification of primary and secondary hypothyroidism. Primary Congenital Hypothyroidism (CH) occurs in 1 out of every 3,000 to 7,000 infants and is caused by athyroidism and hypoplasia. If infants are screened for this disorder during their first month, then irreversible mental retardation can be prevented through early identification and proper treatment. The state of infant's thyroid can be determined by a T4 and TSH combination-screening program. This is the most effective method for the clinician because secondary hypothyroidism may be missed by some TSH screenings and T4 screenings may miss minimal hyperthyroidism. Before starting therapy, a confirmation test should be performed if an infant is thought to be suffering from marginal or borderline hypothyroidism. These determinations should be performed using serum T3, T4, and TSH. Due to infant age, weight, prematurity and demographic variation concentrations of TSH and T4 have been shown to have some variation. Thus each laboratory must establish its own normal and cut-off values. Yes Biotech Laboratories has developed a kit using a method of collecting blood spot samples on S&S #903 filter paper and ELISA techniques. This kit can quantitatively determine TSH level in neonates sensitively, accurately, safely and reliably. It is an important and practical tool to determine thyroids state of neonates, thus making it possible to prevent against infant mental retardation.
Precautions
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