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TSH elisa kit :: Thyroid Stimulating Hormone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS590037
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Wells  /  $440 +1 FREE 8GB USB
 
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 Product Name   

Thyroid Stimulating Hormone (TSH), ELISA Kit

 Also Known As   

Thyroid Stimulating Hormone (TSH) ultra

 Product Gene Name   

TSH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Specificity    This kit exhibits no detectable cross-reaction with human FSH, LH, Prolactin, or CG. hGH can be detected in this assay.
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 Samples    Serum
 Assay Type    Sandwich
 Sensitivity    The minimal detectable concentration of TSH by this assay is estimated to be 0.05 muIU/mL.
 Intended Uses    This Ultra-sensitive Human TSH ELISA Kit is to be used for the in vitro quantitative determination of human thyroid stimulating hormone (TSH) concentrations in serum. This kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in diagnostic or therapeutic procedures.
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 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TSH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TSH purchase    MBS590037 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyroid Stimulating Hormone (TSH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TSH, with an estimated sensitivity of The minimal detectable concentration of TSH by this assay is estimated to be 0.05 muIU/mL. The ELISA analytical biochemical technique of the MBS590037 kit is based on TSH antibody-TSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TSH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TSH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TSH elisa kit

   Principle of the assay: This ultra-sensitive TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH, if present, will bind to the antibody precoated on the wells. In order to quantitate the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody, specific for TSH are added to each well to "sandwich" the TSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSH and enzymeconjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm. In order to measure the concentration of TSH in the sample this Ultra-sensitive Human TSH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus TSH concentration (muIU/mL). The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: The determination of serum or plasma levels of thyroid-stimulating hormone (TSH, or thyrotropin) is recognized as a sensitive method in the diagnosis of primary and secondary hypothyroidism. Thyroid-stimulating hormone is secreted by the anterior lobe of the pituitary gland and induces the production and release of thyroxine and triiodothyronine from the thyroid gland. It is glycoprotein with a molecular weight of approximately 28,000 Dalton, consisting of two chemically different subunits, alpha and beta. Although the concentration of TSH in the blood is extremely low, it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSHreleasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood. This process is known as a negative feedback mechanism and is responsible for maintaining the proper blood levels of these hormones. Conventional TSH assays are generally accepted as an important tool in the diagnosis of primary and secondary hypothyroidism, but they offer limited clinical utility in the assessment of hyperthyroidism due to lack of sensitivity. This Ultra-sensitive TSH assay offers a sensitivity of 0.05 muIU/mL and hence allows discrimination between hyperthyroid and normal patient populations. TSH and the pituitary glycoproteins: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) have identical alpha chains. The beta chain is distinct but does contain identical amino acid sequences, which can cause considerable cross-reactivity with some polyclonal TSH antisera. The use of monoclonal antibodies in this Ultra-sensitive Human TSH ELISA Kit eliminates this interference, which could result in falsely elevated TSH values in either menopausal or pregnant females, a population whose evaluation of thyroid status is clinically significant.
 Product Categories/Family for TSH elisa kit    ELISA Kits for Human Hormones
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 Typical Testing Data/Standard Curve (for reference only) of TSH elisa kit    TSH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS590037. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TSH. It may not necessarily be applicable to this product.
 NCBI GI #    469236
 NCBI Accession #    AAA24698.1 [Other Products]
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 UniProt Secondary Accession #    Q6QE02; Q83WR9 [Other Products]
 Molecular Weight    148,227 Da
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 NCBI Official Full Name    Tsh
 UniProt Protein Name    Temperature-sensitive hemagglutinin tsh autotransporter
 UniProt Synonym Protein Names   
Autotransporter tsh
 Protein Family    Temperature-sensitive hemagglutinin tsh autotransporter
 UniProt Gene Name    tsh [Similar Products]
 UniProt Entry Name    TSH_ECOLX
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 UniProt Comments for TSH    Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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