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TF elisa kit :: Transferrin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS564061 TF elisa kit
Unit / Price
1x96 Wells  /  $405 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transferrin (TF), ELISA Kit

★Popular Item★
 Also Known As   

Human Transferrin ELISA Kit

 Product Gene Name   

TF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 3; NC_000003.11 (133464977..133497850). Location: 3q22.1
 OMIM    190000
 3D Structure    ModBase 3D Structure for P02787
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 Host    Human
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 Samples    Immunoperoxidase Assay for Determination of Transferrin in Human Samples
 Assay Type    Sandwich
 Intended Uses    The Transferrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in human biological samples.
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TF purchase    MBS564061 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Human Transferrin (TF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TF. The ELISA analytical biochemical technique of the MBS564061 kit is based on TF antibody-TF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Immunoperoxidase Assay for Determination of Transferrin in Human Samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TF elisa kit

   Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-TX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound TX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo-genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TX in the test sample. The quantity of TX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Transferrin (TX) is a metal-combining protein that reversibly binds to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, Transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.
 Product Categories/Family for TF elisa kit    ELISA Kit
Sample Manual Insert of MBS564061. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product.
 NCBI GI #    248648
 NCBI GeneID    7018
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 UniProt Primary Accession #    P02787 [Other Products]
 UniProt Secondary Accession #    O43890; Q1HBA5; Q9NQB8; Q9UHV0 [Other Products]
 UniProt Related Accession #    P02787; Q06AH7; Q53H26; Q9UHS1 [Other Products]
 Molecular Weight    77,064 Da [Similar Products]
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 NCBI Official Full Name    transferrin
 NCBI Official Synonym Full Names    transferrin
 NCBI Official Symbol    TF [Similar Products]
 NCBI Official Synonym Symbols   
PRO1557; PRO2086; DKFZp781D0156
[Similar Products]
 NCBI Protein Information    serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin
 UniProt Protein Name    Serotransferrin
 UniProt Synonym Protein Names   
Beta-1 metal-binding globulin; Siderophilin
 Protein Family    Transferrin
 UniProt Gene Name    TF [Similar Products]
 UniProt Entry Name    TRFE_HUMAN
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 NCBI Summary for TF    This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq]
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 UniProt Comments for TF    TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; perinuclear region of cytoplasm; endocytic vesicle; late endosome; apical plasma membrane; basal plasma membrane; endosome membrane; vesicle

Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding

Biological Process: retinal homeostasis; platelet activation; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport

Disease: Atransferrinemia
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 Research Articles on TF    1. CSF total-Tf, a new biomarker, when combined with the current biomarker total-tau, is a reliable pre-mortem diagnostic test for Sporadic Creutzfeldt-Jakob-disease .
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TF elisa kitPathways associated with TF elisa kit
 Reference Product  PubMed Publications
 TFR2 elisa kit  >32 publications with TF and TFR2
 TFRC elisa kit  >13 publications with TF and TFRC
 IGFBP3 elisa kit  >3 publications with TF and IGFBP3
 IGFBP1 elisa kit  >2 publications with TF and IGFBP1
 IGFBP4 elisa kit  >1 publications with TF and IGFBP4
 Products by Pathway  Pathway Diagram
 EPHB Forward Signaling Pathway antibodies  EPHB Forward Signaling Pathway Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
Diseases associated with TF elisa kitOrgans/Tissues associated with TF elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1449 publications with TF and Neoplasms
 Inflammation Antibodies  >605 publications with TF and Inflammation
 Leukemia Antibodies  >457 publications with TF and Leukemia
 Carcinoma Antibodies  >426 publications with TF and Carcinoma
 Blood Coagulation Disorders Antibodies  >294 publications with TF and Blood Coagulation Disorders
 Myocardial Ischemia Antibodies  >271 publications with TF and Myocardial Ischemia
 Metabolic Diseases Antibodies  >258 publications with TF and Metabolic Diseases
 Lung Diseases Antibodies  >235 publications with TF and Lung Diseases
 Atherosclerosis Antibodies  >235 publications with TF and Atherosclerosis
 Drug Toxicity Antibodies  >198 publications with TF and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >666 publications with TF and Brain
 Liver Antibodies  >500 publications with TF and Liver
 Muscle Antibodies  >434 publications with TF and Muscle
 Eye Antibodies  >121 publications with TF and Eye
 Uncharacterized Tissue Antibodies  >7 publications with TF and Uncharacterized Tissue
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