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PLAT elisa kit :: tPA total antigen assay ELISA Kit

Scan QR to view Datasheet Catalog #    MBS135510
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1 Kit  /  $540 +1 FREE 8GB USB
5 Kits  /  $1,930 +2 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

tPA total antigen assay (PLAT), ELISA Kit

★Popular Item★
 Also Known As   

Human tPA total antigen assay ELISA Kit

 Product Gene Name   

PLAT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 8; NC_000008.10 (42032236..42065194, complement). Location: 8p12
 OMIM    173370
 3D Structure    ModBase 3D Structure for P00750
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 Host    Multiple
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 Specificity    These studies are currently in progress. Please contact us for more information.
 Form/Format    Complete Kit
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 Samples    Human plasma and other biological fluids
 Intra-assay Precision    These studies are currently in progress. Please contact us for more information.
 Inter-assay Precision    These studies are currently in progress. Please contact us for more information.
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 Preparation and Storage    Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLAT purchase    MBS135510 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple tPA total antigen assay (PLAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLAT. The ELISA analytical biochemical technique of the MBS135510 kit is based on PLAT antibody-PLAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PLAT elisa kit

   Intended Uses: This human tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tissue plasminogen activator in human plasma and other biological fluids. For research use only.

Principle of the Assay: Human tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free, latent and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. Color development is proportional to the concentration of tPA in the samples.

Background: tPA is a serine protease that catalyzes the activation of plasminogen to plasmin [1]. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis [2], whereas decreased levels may cause neuronal plasticity and degeneration [3].
 Product Categories/Family for PLAT elisa kit    Tissue Plasminogen Activator (tPA); Human tPA ELISA Kits
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 Typical Testing Data/Standard Curve (for reference only) of PLAT elisa kit    PLAT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS135510. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PLAT. It may not necessarily be applicable to this product.
 NCBI GI #    4505861
 NCBI GeneID    5327
 NCBI Accession #    NP_000921.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000930.3 [Other Products]
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 UniProt Primary Accession #    P00750 [Other Products]
 UniProt Secondary Accession #    Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8 [Other Products]
 UniProt Related Accession #    P00750 [Other Products]
 Molecular Weight    62,917 Da
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 NCBI Official Full Name    tissue-type plasminogen activator isoform 1 preproprotein
 NCBI Official Synonym Full Names    plasminogen activator, tissue
 NCBI Official Symbol    PLAT [Similar Products]
 NCBI Official Synonym Symbols   
TPA; T-PA
[Similar Products]
 NCBI Protein Information    tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
 UniProt Protein Name    Tissue-type plasminogen activator
 Protein Family    Tissue-type plasminogen activator
 UniProt Gene Name    PLAT [Similar Products]
 UniProt Synonym Gene Names    t-PA; t-plasminogen activator; tPA [Similar Products]
 UniProt Entry Name    TPA_HUMAN
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 NCBI Summary for PLAT    This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PLAT    PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.68; Secreted, signal peptide; Protease; Secreted; Cytoskeletal; Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
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 Research Articles on PLAT    1. These results suggest that retained tPA on vascular endothelial cells effectively activated plasminogen to plasmin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLAT elisa kitPathways associated with PLAT elisa kit
 Reference Product  PubMed Publications
 SERPINE1 elisa kit  >21 publications with PLAT and SERPINE1
 PLG elisa kit  >8 publications with PLAT and PLG
 PLAU elisa kit  >6 publications with PLAT and PLAU
 SPARC elisa kit  >4 publications with PLAT and SPARC
 FGA elisa kit  >2 publications with PLAT and FGA
 ANXA2 elisa kit  >2 publications with PLAT and ANXA2
 LRP1 elisa kit  >1 publications with PLAT and LRP1
 HMGB1 elisa kit  >1 publications with PLAT and HMGB1
 CALR elisa kit  >1 publications with PLAT and CALR
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with PLAT elisa kitOrgans/Tissues associated with PLAT elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >25 publications with PLAT and Inflammation
 Arterial Occlusive Diseases Antibodies  >22 publications with PLAT and Arterial Occlusive Diseases
 Thrombosis Antibodies  >18 publications with PLAT and Thrombosis
 Atherosclerosis Antibodies  >18 publications with PLAT and Atherosclerosis
 Cerebral Infarction Antibodies  >13 publications with PLAT and Cerebral Infarction
 Necrosis Antibodies  >13 publications with PLAT and Necrosis
 Stroke Antibodies  >12 publications with PLAT and Stroke
 Myocardial Infarction Antibodies  >12 publications with PLAT and Myocardial Infarction
 Kidney Diseases Antibodies  >9 publications with PLAT and Kidney Diseases
 Embolism and Thrombosis Antibodies  >9 publications with PLAT and Embolism and Thrombosis
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >45 publications with PLAT and Vascular
 Liver Antibodies  >43 publications with PLAT and Liver
 Brain Antibodies  >36 publications with PLAT and Brain
 Heart Antibodies  >35 publications with PLAT and Heart
 Lung Antibodies  >28 publications with PLAT and Lung
 Muscle Antibodies  >24 publications with PLAT and Muscle
 Intestine Antibodies  >17 publications with PLAT and Intestine
 Bone Antibodies  >16 publications with PLAT and Bone
 Embryonic Tissue Antibodies  >15 publications with PLAT and Embryonic Tissue
 Kidney Antibodies  >14 publications with PLAT and Kidney
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