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TLR4 elisa kit :: toll-like receptor 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705466
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

toll-like receptor 4 (TLR4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Soluble Toll-like Receptor 4, sTLR4 ELISA Kit

 Product Gene Name   

TLR4 elisa kit

[Similar Products]
 Product Synonym Gene Name    ARMD10; CD284; TOLL; hToll; homolog of Drosophila toll [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705466 Testing Data    Testing Data PDF
 Host    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse sTLR4. No significant cross-reactivity or interference between mouse sTLR4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TLR4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TLR4 purchase    MBS705466 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mouse toll-like receptor 4 (TLR4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing TLR4, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS705466 kit is based on TLR4 antibody-TLR4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TLR4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TLR4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TLR4 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTLR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTLR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTLR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTLR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of TLR4 elisa kit    TLR4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705466. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TLR4. It may not necessarily be applicable to this product.
 NCBI GI #    260401829
 NCBI Accession #    ACX37356.1 [Other Products]
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 NCBI Official Full Name    TLR4
 Protein Family    Toll-like receptor
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 UniProt Comments for TLR4    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TLR4 elisa kitPathways associated with TLR4 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with TLR4 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with TLR4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with TLR4 and Bone
 Connective Tissue Antibodies  >4 publications with TLR4 and Connective Tissue
 Heart Antibodies  >3 publications with TLR4 and Heart
 Muscle Antibodies  >3 publications with TLR4 and Muscle
 Brain Antibodies  >3 publications with TLR4 and Brain
 Vascular Antibodies  >2 publications with TLR4 and Vascular
 Embryonic Tissue Antibodies  >2 publications with TLR4 and Embryonic Tissue
 Ear Antibodies  >1 publications with TLR4 and Ear
 Kidney Antibodies  >1 publications with TLR4 and Kidney
 Umbilical Cord Antibodies  >1 publications with TLR4 and Umbilical Cord
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