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Ace enzyme :: Angiotensin Converting Enzyme, Rat Enzyme

Scan QR to view Datasheet Catalog #    MBS653765 Ace enzyme
Unit / Price
1 Unit  /  $665 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin Converting Enzyme, Rat (Ace), Enzyme

 Also Known As   

Angiotensin Converting Enzyme, Rat (ACE)

 Product Gene Name   

Ace enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_005109.3 (93922062..93965127). Location: 10q32.1
 3D Structure    ModBase 3D Structure for P47820
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 Host    Rat lung
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 Purity/Purification    Highly purified (> 95% by SDS-PAGE).
 Form/Format    Supplied as a liquid in 100mM PBS, 150mM sodium chloride, pH 7.4 with 0.2mM CHAPS. No preservatives added.
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 Activity    ~7mU/ ug of protein
(One unit will produce 1uMole of hippuric acid or His-Leu from Z-Phe-His-Leu per minute in 0.1M phosphate buffer, 30 mM sodium chloride, at pH 8.3 at 37 degree C).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of Ace enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Ace enzyme

   ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase. ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vaso-depressor. ACE appears to play a key role in regulating vascular tone and remodeling the development of atherosclerotic lesions. Increasing evidence suggests that ACE plays an important role in vascular pathology. Recent studies shows two new functions of this important enzyme: 1) ACE enhances the presentation of endogenous antigens (in particularly HIV 1 gp160-derived peptide p18) to MHC class I-restricted T-lynphocytes (ref. 1); 2) amino-terminal domain of ACE cleaves effectively hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (ref. 2,3) which is involved in the control of hemapoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (ref. 4).
 Product Categories/Family for Ace enzyme    Molecular Biology; MB-Enzymes, Angiotensin Converting (ACE)
 Applications Tested/Suitable for Ace enzyme   

Western Blot (WB), Immunoprecipitation (IP)

 Application Notes for Ace enzyme    Suitable for use as a reference in SDS-PAGE and Western blotting (together with anti-ACE monoclonal), as a reference antigen for immunoprecipitation, and as a source of pure ACE for different enzymatic studies of ACE.
Dilution: Western Blot: 500ng/lane if gel is developed with anti-ACE antibody. Optimal dilutions to be determined by the researcher.
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NCBI/Uniprot data below describe general gene information for Ace. It may not necessarily be applicable to this product.
 NCBI GI #    6978757
 NCBI GeneID    24310
 NCBI Accession #    NP_036676.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012544.1 [Other Products]
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 UniProt Primary Accession #    P47820 [Other Products]
 UniProt Secondary Accession #    Q7TMC6; Q8CFN1; Q9EQM9 [Other Products]
 UniProt Related Accession #    P47820 [Other Products]
 Molecular Weight    150,908 Da [Similar Products]
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 NCBI Official Full Name    angiotensin-converting enzyme
 NCBI Official Synonym Full Names    angiotensin I converting enzyme
 NCBI Official Symbol    Ace [Similar Products]
 NCBI Official Synonym Symbols   
Dcp1; StsRR92
[Similar Products]
 NCBI Protein Information    angiotensin-converting enzyme; kininase II; dipeptidyl carboxypeptidase I; angiotensin 1 converting enzyme 1; angiotensin I converting enzyme 1; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme); angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1)
 UniProt Protein Name    Angiotensin-converting enzyme
 UniProt Synonym Protein Names   
Dipeptidyl carboxypeptidase I; Kininase II
 Protein Family    Acetylcholinesterase
 UniProt Gene Name    Ace [Similar Products]
 UniProt Synonym Gene Names    Dcp1; ACE [Similar Products]
 UniProt Entry Name    ACE_RAT
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 NCBI Summary for Ace    catalyzes the conversion of angiotensin I to angiotensin II; plays a role in regulation of blood pressure [RGD, Feb 2006]
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 UniProt Comments for Ace    Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm

By similarity.

Catalytic activity: Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactor: Binds 2 zinc ions per subunit. Isoform Testis-specific only binds 1 zinc ion per subunit

By similarity.Binds 3 chloride ions per subunit

By similarity.

Subcellular location: Angiotensin-converting enzyme, soluble form: Secreted

By similarity. Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Testis-specific isoform is expressed in spermatocytes, adult testis. Also expressed in brain, kidney, lung, skeletal muscle and heart. Ref.3

Induction: Up-regulated after myocardial infarction. Ref.6

Post-translational modification: Phosphorylated by CK2 on Ser-1306; which allows membrane retention

By similarity.

Sequence similarities: Belongs to the peptidase M2 family.
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 Research Articles on Ace    1. Increased expression of pulmonary ACE contributes to elevated circulating angiotensin II in gestational protein restriction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Ace enzymeDiseases associated with Ace enzyme
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin - Angiotensin System Pathway antibodies  Renin - Angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >13366 publications with Ace and Cardiovascular Diseases
 Hypertension Antibodies  >8591 publications with Ace and Hypertension
 Heart Failure Antibodies  >4528 publications with Ace and Heart Failure
 Kidney Diseases Antibodies  >3368 publications with Ace and Kidney Diseases
 Myocardial Infarction Antibodies  >2508 publications with Ace and Myocardial Infarction
 Coronary Artery Disease Antibodies  >2157 publications with Ace and Coronary Artery Disease
 Coronary Disease Antibodies  >2141 publications with Ace and Coronary Disease
 Stroke Antibodies  >1363 publications with Ace and Stroke
 Renal Insufficiency Antibodies  >1280 publications with Ace and Renal Insufficiency
 Respiratory Tract Diseases Antibodies  >1240 publications with Ace and Respiratory Tract Diseases
Organs/Tissues associated with Ace enzyme
 Organ/Tissue Name  Pubmed Publications
 Artery Antibodies  >3460 publications with Ace and Artery
 Lung Antibodies  >1546 publications with Ace and Lung
 Brain Antibodies  >1246 publications with Ace and Brain
 Eye Antibodies  >233 publications with Ace and Eye
 Testis Antibodies  >223 publications with Ace and Testis
 Connective Tissue Antibodies  >183 publications with Ace and Connective Tissue
 Embryonic Tissue Antibodies  >138 publications with Ace and Embryonic Tissue
 Small Intestine Antibodies  >120 publications with Ace and Small Intestine
 Ovary Antibodies  >114 publications with Ace and Ovary
 Colon Antibodies  >99 publications with Ace and Colon
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