Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Enzyme rightarrow ENPP1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • YAP Antibody
 • pro-Brain Natriuretic Peptide, N-terminal (NT-proBNP) (a.a. ... Antibody
 • Interleukin-18, Mouse (IL-18) ELISA Kit
 • Protein tyrosine phosphatase (PTP) ELISA Kit
 • Total bile acid (TBA) ELISA Kit
 • Putative phosphotransferase CLK_3095 (CLK_3095) Recombinant Protein
 • O3FAR1 - GPR120 Antibody
 • TLR2 Antibody
 • TAK1-MAP3K7 (MAP3K7 ) Antibody
 • NRG3 Antibody
 • 40S ribosomal protein S6 (RPS6) ELISA Kit
 • tripartite motif-containing 9 (TRIM9) ELISA Kit
 • Troponin T Type 2, Cardiac (TNNT2) CLIA Kit
 • Cysteine and glycine-rich protein 3 (CSRP3) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ENPP1 enzyme :: Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 Enzyme

Scan QR to view Datasheet Catalog #    MBS653632 ENPP1 enzyme
Unit / Price
0.1 mg  /  $400 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 (ENPP1), Enzyme

★Popular Item★
 Also Known As   

Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 (ENPP1, PC1, Plasma Cell Membrane Glycoprotein) (Control Peptide)

 Product Gene Name   

ENPP1 enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: 6; NC_000006.11 (132129156..132216295). Location: 6q22-q23
 OMIM    125853
 3D Structure    ModBase 3D Structure for P22413
Section Bar
Section Bar
 Purity/Purification    Highly Purified
 Form/Format    Supplied as a lyophilized powder from acetonitrile/H2O. Reconstitution: Reconstitute with 200ul sterile ddH2O
 Concentration    ~0.5 mg/ml (after reconstitution) (lot specific)
Section Bar
 Source    Synthetic
Section Bar
 Preparation and Storage    Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the orginal vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of ENPP1 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for ENPP1 enzyme

   C-KTHLPTFSQED, from the C Terminus of human ENPP1. Control peptide corresponding to polyclonal antibody, Catalog#MBS623475, goat x human.
Peptide Blocking:
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ug pellet. When peptides are reconstituted in 200ul water, the concentration will be 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
 Product Categories/Family for ENPP1 enzyme    Molecular Biology; MB-Enzymes, Phosphodiesterase
 Applications Tested/Suitable for ENPP1 enzyme   

Suitable for use in ELISA and Antibody blocking
Other applications not tested

 Application Notes for ENPP1 enzyme    Optimal dilutions to be determined by the researcher
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ENPP1. It may not necessarily be applicable to this product.
 NCBI GI #    170650661
 NCBI GeneID    5167
 NCBI Accession #    NP_006199.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006208.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P22413 [Other Products]
 UniProt Secondary Accession #    Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3 [Other Products]
 UniProt Related Accession #    P22413 [Other Products]
Table BarTOPTable Bar
 NCBI Official Full Name    ectonucleotide pyrophosphatase/phosphodiesterase family member 1
 NCBI Official Synonym Full Names    ectonucleotide pyrophosphatase/phosphodiesterase 1
 NCBI Official Symbol    ENPP1 [Similar Products]
 NCBI Official Synonym Symbols   
M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; PDNP1
[Similar Products]
 NCBI Protein Information    ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1; membrane component chromosome 6 surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; membrane component, chromosome 6, surface marker 1
 UniProt Protein Name    Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
 UniProt Synonym Protein Names   
Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1
 Protein Family    Ectonucleotide pyrophosphatase/phosphodiesterase family
 UniProt Gene Name    ENPP1 [Similar Products]
 UniProt Synonym Gene Names    M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase [Similar Products]
 UniProt Entry Name    ENPP1_HUMAN
Section Bar
 NCBI Summary for ENPP1    This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for ENPP1    Function: By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Ref.10

Catalytic activity: Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.A dinucleotide + H2O = 2 mononucleotides.

Cofactor: Binds 2 divalent metal cations per subunit

By similarity.

Enzyme regulation: At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis.

Subunit structure: The secreted form is monomeric

By similarity. Homodimer; disulfide-linked. Interacts with INSR. Ref.10

Subcellular location: Cell membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Secreted

By similarity. Note: The proteolytically processed form is secreted

By similarity. Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side. Ref.11 Ref.12

Tissue specificity: Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. Ref.9

Domain: The di-leucine motif is required for basolateral targeting in epithelial cells, and for targeting to matrix vesicles derived from mineralizing cells

By similarity.

Post-translational modification: Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.N-glycosylated.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.The interchain disulfide bonding resides in the transmembrane segment

By similarity.A secreted form is produced through cleavage at Lys-103 by intracellular processing

By similarity.

Involvement in disease: Ossification of the posterior longitudinal ligament of the spine (OPLL) [MIM:602475]: A calcification of the posterior longitudinal ligament of the spinal column, usually at the level of the cervical spine. Patients with OPLL frequently present with a severe myelopathy that can lead to tetraparesis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16Arterial calcification of infancy, generalized, 1 (GACI1) [MIM:208000]: A severe autosomal recessive disorder characterized by calcification of the internal elastic lamina of muscular arteries and stenosis due to myointimal proliferation. The disorder is often fatal within the first 6 months of life because of myocardial ischemia resulting in refractory heart failure.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17 Ref.18 Ref.20 Ref.24Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.19Hypophosphatemic rickets, autosomal recessive, 2 (ARHR2) [MIM:613312]: A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.21 Ref.22

Sequence similarities: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.Contains 2 SMB (somatomedin-B) domains.

Caution: It is uncertain whether Met-1 or Met-53 is the initiator.

Sequence caution: The sequence AAA63237.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH59375.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAA02054.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Table BarTOPTable Bar
 Research Articles on ENPP1    1. mutation in GOLGB1 (Y1212C) and another mutation in ENPP1 (K173Q) were confirmed as having significant associations with a decreased risk for stroke
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ENPP1 enzymePathways associated with ENPP1 enzyme
 Reference Product  PubMed Publications
 NT5E enzyme  >4 publications with ENPP1 and NT5E
 CD38 enzyme  >1 publications with ENPP1 and CD38
 Products by Pathway  Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 NAD Salvage Pathway II antibodies  NAD Salvage Pathway II Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
Diseases associated with ENPP1 enzymeOrgans/Tissues associated with ENPP1 enzyme
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >59 publications with ENPP1 and Diabetes Mellitus, Type 2
 Obesity Antibodies  >48 publications with ENPP1 and Obesity
 Arterial calcification of infancy Antibodies  >24 publications with ENPP1 and Arterial calcification of infancy
 Kidney Diseases Antibodies  >18 publications with ENPP1 and Kidney Diseases
 Disease Models, Animal Antibodies  >8 publications with ENPP1 and Disease Models, Animal
 Liver Diseases Antibodies  >8 publications with ENPP1 and Liver Diseases
 Hyperglycemia Antibodies  >6 publications with ENPP1 and Hyperglycemia
 Inflammation Antibodies  >5 publications with ENPP1 and Inflammation
 Fatty Liver Antibodies  >4 publications with ENPP1 and Fatty Liver
 Fibrosis Antibodies  >4 publications with ENPP1 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >45 publications with ENPP1 and Bone
 Blood Antibodies  >41 publications with ENPP1 and Blood
 Heart Antibodies  >19 publications with ENPP1 and Heart
 Muscle Antibodies  >15 publications with ENPP1 and Muscle
 Connective Tissue Antibodies  >13 publications with ENPP1 and Connective Tissue
 Liver Antibodies  >13 publications with ENPP1 and Liver
 Kidney Antibodies  >13 publications with ENPP1 and Kidney
 Skin Antibodies  >8 publications with ENPP1 and Skin
 Embryonic Tissue Antibodies  >5 publications with ENPP1 and Embryonic Tissue
 Eye Antibodies  >3 publications with ENPP1 and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions