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MPO enzyme :: Myeloperoxidase, Neutrophil Enzyme

Scan QR to view Datasheet Catalog #    MBS653922 MPO enzyme
Unit / Price
0.1 mg  /  $255 +1 FREE 8GB USB
1 mg  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myeloperoxidase, Neutrophil (MPO), Enzyme

 Also Known As   

Myeloperoxidase, Human, Neutrophil (MPO, pANCA)

 Product Gene Name   

MPO enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 17; NC_000017.10 (56347217..56358296, complement). Location: 17q23.1
 OMIM    254600
 3D Structure    ModBase 3D Structure for P05164
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 Host    Human neutrophils
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 Purity/Purification    Highly Purified
~90% (SDS-PAGE). No detectable amounts of lactoferrin or Cathepsin G.
 Form/Format    Supplied as a greenish liquid in sodium acetate, pH 6.0.
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 CAS Number    9003-99-0
 Unit Definition    One unit of MPO will consume 1uM of hydrogen peroxide and produce 0.25uM of tetraguaiacol per minute at pH 7, 25ºC.
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 Preparation and Storage    May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of MPO enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for MPO enzyme

   In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive. Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.q., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkin's lymphomas of T-or B-cell type, and Hodgkin's disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues. MPO is useful in differentiating between myeloid and lymphoid leukemias.
 Product Categories/Family for MPO enzyme    Molecular Biology; MB-Enzymes, Myeloperoxidase
 Applications Tested/Suitable for MPO enzyme   

ELISA (EL/EIA)

 Application Notes for MPO enzyme    Suitable for use in ELISA.
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NCBI/Uniprot data below describe general gene information for MPO. It may not necessarily be applicable to this product.
 NCBI GI #    4557759
 NCBI GeneID    4353
 NCBI Accession #    NP_000241.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000250.1 [Other Products]
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 UniProt Primary Accession #    P05164 [Other Products]
 UniProt Secondary Accession #    Q14862; Q4PJH5; Q9UCL7; A1L4B8 [Other Products]
 UniProt Related Accession #    P05164 [Other Products]
 Molecular Weight    145kD; 145kD, Composed of 2 identical subunits of 72.5kD. Each subunit is joined by a single disulfide bridge yielding peptides of 59 and 13.5kD. [Similar Products]
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 NCBI Official Full Name    myeloperoxidase
 NCBI Official Synonym Full Names    myeloperoxidase
 NCBI Official Symbol    MPO [Similar Products]
 NCBI Protein Information    myeloperoxidase
 UniProt Protein Name    Myeloperoxidase
 Protein Family    Myeloperoxidase
 UniProt Gene Name    MPO [Similar Products]
 UniProt Synonym Gene Names    MPO [Similar Products]
 UniProt Entry Name    PERM_HUMAN
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 NCBI Summary for MPO    Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MPO    Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Catalytic activity: Cl- + H2O2 + H+ = HClO + H2O.Cl- + H2O2 = HOCl + 2 H2O.

Cofactor: Binds 1 calcium ion per monomer.Binds 1 heme B (iron-protoporphyrin IX) group covalently per monomer.

Subunit structure: Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Ref.13 Ref.21

Subcellular location: Lysosome.

Involvement in disease: Myeloperoxidase deficiency (MPOD) [MIM:254600]: A disorder characterized by decreased myeloperoxidase activity in neutrophils and monocytes that results in disseminated candidiasis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.24 Ref.25 Ref.26 Ref.27 Ref.28

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.
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 Research Articles on MPO    1. Data suggest that MPO does not have several binding sites as found for other heme-proteins but rather MPO has a lone binding site/binding region that accepts several ligands (substrates and inhibitors) to mediate its locally rich pi-pi environment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MPO enzymePathways associated with MPO enzyme
 Reference Product  PubMed Publications
 PRTN3 enzyme  >187 publications with MPO and PRTN3
 MYB enzyme  >10 publications with MPO and MYB
 JAK2 enzyme  >4 publications with MPO and JAK2
 ALB enzyme  >4 publications with MPO and ALB
 ELANE enzyme  >1 publications with MPO and ELANE
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
 IL23-mediated Signaling Events Pathway antibodies  IL23-mediated Signaling Events Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Amb2 Integrin Signaling Pathway antibodies  Amb2 Integrin Signaling Pathway Diagram
Diseases associated with MPO enzymeOrgans/Tissues associated with MPO enzyme
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2221 publications with MPO and Inflammation
 Cardiovascular Diseases Antibodies  >1810 publications with MPO and Cardiovascular Diseases
 Necrosis Antibodies  >1256 publications with MPO and Necrosis
 Lung Diseases Antibodies  >1064 publications with MPO and Lung Diseases
 Reperfusion Injury Antibodies  >764 publications with MPO and Reperfusion Injury
 Kidney Diseases Antibodies  >581 publications with MPO and Kidney Diseases
 Edema Antibodies  >530 publications with MPO and Edema
 Hemorrhage Antibodies  >400 publications with MPO and Hemorrhage
 Heart Diseases Antibodies  >378 publications with MPO and Heart Diseases
 Wounds and Injuries Antibodies  >342 publications with MPO and Wounds and Injuries
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4484 publications with MPO and Blood
 Lung Antibodies  >1316 publications with MPO and Lung
 Kidney Antibodies  >633 publications with MPO and Kidney
 Brain Antibodies  >529 publications with MPO and Brain
 Bone Antibodies  >350 publications with MPO and Bone
 Bone Marrow Antibodies  >285 publications with MPO and Bone Marrow
 Eye Antibodies  >85 publications with MPO and Eye
 Lymph Node Antibodies  >76 publications with MPO and Lymph Node
 Thyroid Antibodies  >69 publications with MPO and Thyroid
 Embryonic Tissue Antibodies  >37 publications with MPO and Embryonic Tissue
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