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PLAU enzyme :: Urokinase Urine Enzyme

Scan QR to view Datasheet Catalog #    MBS653743 PLAU enzyme
Unit / Price
0.1 mg  /  $355 +1 FREE 8GB USB
1 mg  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Urokinase Urine (PLAU), Enzyme

 Also Known As   

Urokinase, Human Urine

 Product Gene Name   

PLAU enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_000010.10 (75670862..75677259). Location: 10q22.2
 OMIM    191840
 3D Structure    ModBase 3D Structure for P00749
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 Host    Human urine
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 Purity/Purification    Highly Purified
90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
 Form/Format    Supplied as a lyophilized powder from PBS. Reconstitute with sterile ddH2O to >0.1mg/ml.
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 Activity    1nM UK will cause a change in absorbance of 0.001 at 405nm in 1 minute at RT in 100ul 0.05M Tris-HCl, 0.1M sodium chloride, pH 7.4, using S2444 as the substrate.
 Specific Activity    hUK conforms an activity greater than 68,000IU/mg.
 Contaminants    Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of PLAU enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PLAU enzyme

   Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture. UK is a two-chain glycoprotein containing 411 amino acids with 12 dissulfide bonds. Its molecular weight is 54kD.
 Product Categories/Family for PLAU enzyme    Molecular Biology; MB-Enzymes
NCBI/Uniprot data below describe general gene information for PLAU. It may not necessarily be applicable to this product.
 NCBI GI #    254763341
 NCBI GeneID    5328
 NCBI Accession #    P00749.2 [Other Products]
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 UniProt Primary Accession #    P00749 [Other Products]
 UniProt Secondary Accession #    Q15844; Q16618; Q53XS3; Q5SWW9; Q969W6; B4DPZ2 [Other Products]
 UniProt Related Accession #    P00749 [Other Products]
 Molecular Weight    48,507 Da [Similar Products]
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 NCBI Official Full Name    Urokinase-type plasminogen activator
 NCBI Official Synonym Full Names    plasminogen activator, urokinase
 NCBI Official Symbol    PLAU [Similar Products]
 NCBI Official Synonym Symbols   
ATF; QPD; UPA; URK; u-PA; BDPLT5
[Similar Products]
 NCBI Protein Information    urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary
 UniProt Protein Name    Urokinase-type plasminogen activator
 Protein Family    Urokinase-type plasminogen activator
 UniProt Gene Name    PLAU [Similar Products]
 UniProt Synonym Gene Names    U-plasminogen activator; uPA [Similar Products]
 UniProt Entry Name    UROK_HUMAN
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 NCBI Summary for PLAU    This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
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 UniProt Comments for PLAU    Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation: Inhibited by SERPINA5. Ref.17 Ref.22

Subunit structure: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR. Ref.20 Ref.21

Subcellular location: Secreted.

Tissue specificity: Expressed in the prostate gland and prostate cancers. Ref.23

Post-translational modification: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Involvement in disease: Quebec platelet disorder (QPD) [MIM:601709]: An autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.24

Pharmaceutical use: Available under the name Abbokinase (Abbott). Used in Pulmonary Embolism (PE) to initiate fibrinolysis. Clinically used for therapy of thrombolytic disorders.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 EGF-like domain.Contains 1 kringle domain.Contains 1 peptidase S1 domain.
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 Research Articles on PLAU    1. In conclusion, our data suggest that radiation-mediated hypomethylation triggers uPA expression in meningioma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLAU enzymePathways associated with PLAU enzyme
 Reference Product  PubMed Publications
 PLAUR enzyme  >16 publications with PLAU and PLAUR
 SERPINE1 enzyme  >12 publications with PLAU and SERPINE1
 MMP9 enzyme  >11 publications with PLAU and MMP9
 SERPINB2 enzyme  >7 publications with PLAU and SERPINB2
 FN1 enzyme  >6 publications with PLAU and FN1
 EGFR enzyme  >2 publications with PLAU and EGFR
 PLG enzyme  >1 publications with PLAU and PLG
 VLDLR enzyme  >1 publications with PLAU and VLDLR
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
Diseases associated with PLAU enzymeOrgans/Tissues associated with PLAU enzyme
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with PLAU and Inflammation
 Adenocarcinoma Antibodies  >15 publications with PLAU and Adenocarcinoma
 Alzheimer Disease Antibodies  >14 publications with PLAU and Alzheimer Disease
 Neoplasm Invasiveness Antibodies  >10 publications with PLAU and Neoplasm Invasiveness
 Prostatic Neoplasms Antibodies  >8 publications with PLAU and Prostatic Neoplasms
 Neoplasm Metastasis Antibodies  >7 publications with PLAU and Neoplasm Metastasis
 Stomach Neoplasms Antibodies  >5 publications with PLAU and Stomach Neoplasms
 Pancreatic Neoplasms Antibodies  >4 publications with PLAU and Pancreatic Neoplasms
 Hemorrhage Antibodies  >3 publications with PLAU and Hemorrhage
 Necrosis Antibodies  >2 publications with PLAU and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >28 publications with PLAU and Blood
 Lung Antibodies  >12 publications with PLAU and Lung
 Brain Antibodies  >10 publications with PLAU and Brain
 Bone Antibodies  >9 publications with PLAU and Bone
 Prostate Antibodies  >9 publications with PLAU and Prostate
 Vascular Antibodies  >9 publications with PLAU and Vascular
 Liver Antibodies  >7 publications with PLAU and Liver
 Placenta Antibodies  >7 publications with PLAU and Placenta
 Stomach Antibodies  >6 publications with PLAU and Stomach
 Skin Antibodies  >6 publications with PLAU and Skin
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