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SERPINA1 enzyme :: a1-Antichymotrypsin Enzyme

Scan QR to view Datasheet Catalog #    MBS653849 SERPINA1 enzyme
Unit / Price
1 mg  /  $820 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

a1-Antichymotrypsin (SERPINA1), Enzyme

 Also Known As   

a1-Antichymotrypsin, Human (AACT, Alpha1-ACT)

 Product Gene Name   

SERPINA1 enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 14; NC_000014.8 (94843084..94857029, complement). Location: 14q32.1
 OMIM    107400
 3D Structure    ModBase 3D Structure for P01009
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 Host    Pooled human serum or plasma
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 Purity/Purification    Highly Purified
98% No contaminants detected. Single band by SDS-PAGE, IEP, and/or RID.
 Form/Format    Lyophilized from 0.02M Tris, 0.2M sodium chloride, pH 7.5.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of SERPINA1 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for SERPINA1 enzyme

   Alpha1-Antichymotrypsin (AACT) is a serine proteinase inhibitor and minor acute phase protein with a molecular weight of ~68kD, occurring in serum at 250-500mg/l. AACT appears to inhibit chymotrypsin specifically.
 Product Categories/Family for SERPINA1 enzyme    Molecular Biology; MB-Enzymes
 Applications Tested/Suitable for SERPINA1 enzyme   

ELISA (EL/EIA)

 Application Notes for SERPINA1 enzyme    Suitable for use in ELISA.
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NCBI/Uniprot data below describe general gene information for SERPINA1. It may not necessarily be applicable to this product.
 NCBI GI #    189163542
 NCBI GeneID    5265
 NCBI Accession #    NP_001121179.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001127707.1 [Other Products]
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 UniProt Primary Accession #    P01009 [Other Products]
 UniProt Secondary Accession #    Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8 [Other Products]
 UniProt Related Accession #    P01009 [Other Products]
 Molecular Weight    ~68kD [Similar Products]
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 NCBI Official Full Name    alpha-1-antitrypsin
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
 NCBI Official Symbol    SERPINA1 [Similar Products]
 NCBI Official Synonym Symbols   
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
[Similar Products]
 NCBI Protein Information    alpha-1-antitrypsin; serpin A1; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
 UniProt Protein Name    Alpha-1-antitrypsin
 UniProt Synonym Protein Names   
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
 Protein Family    Alpha-1-antitrypsin
 UniProt Gene Name    SERPINA1 [Similar Products]
 UniProt Synonym Gene Names    AAT; PI; SPAAT [Similar Products]
 UniProt Entry Name    A1AT_HUMAN
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 NCBI Summary for SERPINA1    The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SERPINA1    Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Ref.17 Ref.18 Ref.24Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). Ref.17 Ref.18 Ref.24

Subcellular location: Secreted Ref.24. Short peptide from AAT: Secreted › extracellular space › extracellular matrix Ref.24.

Tissue specificity: Plasma.

Domain: The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

Post-translational modification: N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Ref.19 Ref.26 Ref.34Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.

Polymorphism: The sequence shown is that of the M1V allele which is the most common form of PI (44 to 49%). Other frequent alleles are: M1A 20 to 23%; M2 10 to 11%; M3 14 to 19%.

Involvement in disease: Alpha-1-antitrypsin deficiency (A1ATD) [MIM:613490]: A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.59 Ref.61 Ref.63

Miscellaneous: The aberrant form is found in the plasma of chronic smokers, and persists after smoking is ceased. It can still be found ten years after smoking has ceased.

Sequence similarities: Belongs to the serpin family.

Sequence caution: The sequence CAD62334.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD62585.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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 Research Articles on SERPINA1    1. Metabolism and metabolic burden by alpha1-antitrypsin production in human AGE1.HN cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SERPINA1 enzymeDiseases associated with SERPINA1 enzyme
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >111 publications with SERPINA1 and Lung Diseases
 alpha 1-Antitrypsin Deficiency Antibodies  >109 publications with SERPINA1 and alpha 1-Antitrypsin Deficiency
 Liver Diseases Antibodies  >103 publications with SERPINA1 and Liver Diseases
 Pulmonary Disease, Chronic Obstructive Antibodies  >39 publications with SERPINA1 and Pulmonary Disease, Chronic Obstructive
 Pulmonary Emphysema Antibodies  >32 publications with SERPINA1 and Pulmonary Emphysema
 Inflammation Antibodies  >29 publications with SERPINA1 and Inflammation
 Liver Cirrhosis Antibodies  >25 publications with SERPINA1 and Liver Cirrhosis
 Cardiovascular Diseases Antibodies  >25 publications with SERPINA1 and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >20 publications with SERPINA1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >17 publications with SERPINA1 and Liver Neoplasms
Organs/Tissues associated with SERPINA1 enzyme
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >99 publications with SERPINA1 and Liver
 Blood Antibodies  >87 publications with SERPINA1 and Blood
 Lung Antibodies  >59 publications with SERPINA1 and Lung
 Skin Antibodies  >13 publications with SERPINA1 and Skin
 Vascular Antibodies  >10 publications with SERPINA1 and Vascular
 Heart Antibodies  >10 publications with SERPINA1 and Heart
 Kidney Antibodies  >9 publications with SERPINA1 and Kidney
 Bone Antibodies  >8 publications with SERPINA1 and Bone
 Pancreas Antibodies  >7 publications with SERPINA1 and Pancreas
 Intestine Antibodies  >6 publications with SERPINA1 and Intestine
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