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ApoMark Apoptosis Kit IHC Kit

Scan QR to view Datasheet Catalog #    MBS396367 ApoMark Apoptosis Kit ihc kit
Unit / Price
30 Tests  /  $305 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ApoMark Apoptosis Kit, IHC Kit

 Also Known As   

ApoMark Apoptosis Kit

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
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 Samples    Paraffi n-embedded tissue sections, tissue cryosections, and in cell preparations fi xed on slides.
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 Preparation and Storage    Store kit at -20 degree C until first use. Store at 4-8 degree C thereafter.
 Other Notes    Small volumes of ApoMark Apoptosis Kit ihc kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for ApoMark Apoptosis Kit ihc kit

   Intended Uses||ApoMark™ Apoptosis Detection Kit is a nonisotopic system for the labeling of DNA breaks in apoptotic cell nuclei in paraffi n-embedded tissue sections, tissue cryosections, and in cell preparations fi xed on slides.

Principle of the Assay: ApopMark™ Apoptosis Detection Kit allows the recognition of apoptotic nuclei in paraffi n-embedded tissue sections, frozen tissue section, or in preparations of single cell suspensions fi xed on slides. In this assay Terminal deoxynucleotidyl Transferase (TdT) binds to exposed 3'-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of biotin-labeled deoxynucleotides. Biotinylated nucleotides are detected using a streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine (DAB) reacts with the HRP labeled sample to generate an insoluble colored (brown) substrate at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.

Background/Introduction: Cell death occurs by two major mechanisms, necrosis and apoptosis. Apoptsis is also known as programmed cell death or ankoikis (a form of apoptosis which is induced by anchorage-dependent cells detaching from the surrounding extracellular matrix). Apoptosis leads to the elimination of cells without releasing harmful substances into the surrounding area. Too little or too much apoptosis plays a role in a great many diseases. When apoptosis functions inappropriately, cells that should be eliminated survive and potentially become immortal, as in cancer or leukemia. When apoptosis works overly well, too many cells may 'die' and the result may be grave tissue damage. This is the case in stroke and neurodegenerative disorders such as Alzheimer, Huntington and Parkinson diseases. The term 'apoptosis' refers only to the structural changes a cell goes through during the process of programmed cell death and not to the process itself. Classical necrotic cell death occurs due to noxious injury or trauma to the cell while apoptosis is an energy dependant mechanism that takes place during normal cell development. While necrotic cell death results in cell lysis, cellular apoptosis is characterized morphologically by cell shrinkage, nuclear pyknosis, chromatin condensation, and blebbing of the plasma membrane. Apoptosis is the result of a cascade of molecular and biochemical events involving endogenous endonucleases that cleave DNA into the prototypical 'ladder of DNA fragments' that may be visualized in agarose gels. Observation of oligonucleosomal DNA fragments by DNA laddering has long been the most acceptable and only available assay for the detection of apoptosis. ApoMark™ assay exploits the fact that apoptotic endonucleases not only affect cellular DNA by producing the classical DNA ladder but also generate free 3'-OH groups at the ends of these DNA fragments. These free 3'-OH groups are end-labeled by the ApoMark™ Apoptosis Detection Kit allowing for the detection of apoptotic cells using a molecular biology-based, endlabeling technique.
 Product Categories/Family for ApoMark Apoptosis Kit ihc kit    Immunohistochemistry Kit
 Applications Tested/Suitable for ApoMark Apoptosis Kit ihc kit   

Apoptosis Quantitation

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Sample Manual Insert of MBS396367
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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