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Casp1 kit :: CASPASE-1 Kit

Scan QR to view Datasheet Catalog #    MBS239202
Testing Data #1
Unit / Price
100 Tests  /  $900 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CASPASE-1 (Casp1), Kit

 Also Known As   

FAM FLICA CASPASE-1 KIT

 Product Gene Name   

Casp1 kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
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 Form/Format    Kit
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 Reagents In The Kits    4 vials of FAM-YVAD-FMK FLICA Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst Stain, 1 mL
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 Preparation and Storage    Store the unopened kit (and each unopened component) at 4 degree C until the expiration date.
Protect the FLICA reagent from light at all times.
Once reconstituted, the 150X FLICA stock should be stored at -20 degree C protected from light.
Shelf Life: The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.
 Product Note    Our Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Casp1 kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Casp1 kit

   Description: The FAM FLICA Caspase 1 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
Test Principle: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
 Applications Tested/Suitable for Casp1 kit   

Flow cytometry (FC/FACS), Immunofluorescence (IF)

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 Testing Data #1 of Casp1 kit    Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases
Casp1 kit Testing Data #1 image
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NCBI/Uniprot data below describe general gene information for Casp1. It may not necessarily be applicable to this product.
 NCBI GI #    31542341
 NCBI GeneID    25166
 NCBI Accession #    NP_036894.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012762.2 [Other Products]
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 UniProt Related Accession #    P43527 [Other Products]
 Molecular Weight    45,576 Da [Similar Products]
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 NCBI Official Full Name    caspase-1
 NCBI Official Synonym Full Names    caspase 1
 NCBI Official Symbol    Casp1 [Similar Products]
 NCBI Official Synonym Symbols   
Ice; Il1bc
[Similar Products]
 NCBI Protein Information    caspase-1; CASP-1; IL-1 beta-converting enzyme; IL-1BC; Interleukin 1beta converting enzyme; interleukin 1, beta, convertase; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; p45
 UniProt Protein Name    Caspase-1
 UniProt Synonym Protein Names   
Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10
 Protein Family    Caspase
 UniProt Gene Name    Casp1 [Similar Products]
 UniProt Synonym Gene Names    Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme [Similar Products]
 UniProt Entry Name    CASP1_RAT
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 NCBI Summary for Casp1    apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
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 UniProt Comments for Casp1    CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)

Protein type: Apoptosis; Protease; EC 3.4.22.36
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 Research Articles on Casp1    1. Caspase-1 activation plays an important role in the development of inflammation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Casp1 kitPathways associated with Casp1 kit
 Reference Product  PubMed Publications
 Nlrp3 kit  >801 publications with Casp1 and Nlrp3
 Il18 kit  >536 publications with Casp1 and Il18
 Pycard kit  >224 publications with Casp1 and Pycard
 Il1b kit  >74 publications with Casp1 and Il1b
 Il33 kit  >17 publications with Casp1 and Il33
 Rela kit  >13 publications with Casp1 and Rela
 Naip2 kit  >8 publications with Casp1 and Naip2
 Nfkb1 kit  >4 publications with Casp1 and Nfkb1
 Nfkb2 kit  >2 publications with Casp1 and Nfkb2
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Organs/Tissues associated with Casp1 kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >228 publications with Casp1 and Lung
 Spleen Antibodies  >97 publications with Casp1 and Spleen
 Eye Antibodies  >94 publications with Casp1 and Eye
 Colon Antibodies  >68 publications with Casp1 and Colon
 Adipose Tissue Antibodies  >36 publications with Casp1 and Adipose Tissue
 Small Intestine Antibodies  >18 publications with Casp1 and Small Intestine
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