View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Artocarpus integrifolia Lectin Catalog # MBS656198 Unit / Price 10 mL / $1,020 +1 FREE 8GB USB 5x10 mL / $4,425 +5 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Artocarpus integrifolia, Lectin Full Product Name Artocarpus integrifolia (Jacalin, Jackfruit) (Agarose) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Purity/Purification Molecular Biology Grade Form/Format Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 20mM galactose, 20mM lactose, 0.08% sodium azide. Labeled with Agarose. Activity 2x10e7 Binding capacity 4mg asialo-fetuin/ml of gel 4mg monomeric IgA/ml of gel Inhibiting/Eluting Sugar 800mM galactose or 100mM melibiose 4mg lectin/ml gel Preparation and Storage May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of Artocarpus integrifolia lectin vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Artocarpus integrifolia lectin Jacalin is a lectin composed of four subunits, two of approximately 10,000D and two of 16,000D each. This 50,000D glycoprotein appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (b-1,3) N-acetylgalactosamine. This structure (the so-called "T-antigen") is the oligosaccharide to which peanut agglutinin binds. However, unlike PNA, Jacalin will bind this structure even in a mono- or disialylated form. This lectin has been used to purify human IgA, since no other human immunoglobulin class binds Jacalin. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains. Agarose bound Jacalin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. acalin is a lectin present in the seeds of the Jackfruit, Artocarpus integrifolia. Recent papers 1,2 reported that Jacalin specifically bound human secretory IgA and that this lectin could be used to sepa- rate human IgA from other serum glycoproteins, including other immunoglobulin classes, and that agarose bound Jacalin could be used to separate IgA1 and IgA2. We have recently affinity purified Jacalin. Our preliminary investigation shows it to have a molecular weight of approximately 60,000 with a rather complex subunit structure. In addition to confirming its affinity toward galactose, we have examined the specificity of this lectin toward several glycoproteins. Using glycoproteins of known carbohydrate structure, we can now provide some information on the structural requirements of the oligosaccharide for binding Jacalin. Product Categories/Family for Artocarpus integrifolia lectin Lectins; Lectin Artocarpus integrifolia Applications Tested/Suitable for Artocarpus integrifolia lectin ELISA (EL/EIA) Application Notes for Artocarpus integrifolia lectin Suitable for use in ELISA. Testing Data of Artocarpus integrifolia lectin Small intestine: Jacalin (brown, DAB), Hematoxylin counterstain. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP