View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) Molecular Weight Marker Catalog # MBS355493 Unit / Price 0.1 mL / $225 +1 FREE 8GB USB 5x0.1 mL / $665 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa), Molecular Weight Marker Popular Item Full Product Name Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS355493 Technical Datasheet Technical Datasheet PDF Form/Format 62.5mM Tris-H3PO4 (pH7.5, 25°C), 1mM EDTA , 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol. Features • Molecular weight range - 10-250 kDa• Brightness reference band -70 kDa, 25kDa• Ready to use - Pre-mixed with loading buffer.• Narrow band Introductions Prestained protein ladders/marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins. Prestained Protein Molwcular Weight Marker is a three-color molecular weight standard , contains 9 prestained natural proteins with molecular weights from 10kDa to 250kDa. It has two orange reference bands -70kDa band and 25kDa band. Protocol 1: Thaw the ladder either at room tempeture or at 37°C for a few minutes to disolve precipitated solids thoroughly, Do not boil!2: Mix gently, but thoroughly to ensure that the solution is homogenous.3: Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm.Large SDS-polyacrylamide gel: 10 ul per well, with thickness of 0.75-1.0 mm. Preparation and Storage Store at 4 degree C for 3 months, and at -20 degree C for long term. Other Notes Small volumes of Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) molecular weight marker vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) molecular weight marker Background/Introduction: Polyphenol oxidase is a bifunctional, copper-containing oxidase having catecholase and cresolase activity. It is responsible for browning reactions through the phylogenetic scale. The assay is initiated with the enzymatic hydrolysis of the Catechol by Polyphenol oxidase. The enzyme catalysed reaction products quinone, can be measured at a colorimetric readout at 410 nm. Product Categories/Family for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) molecular weight marker Molecular Weight Marker Application Notes for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) molecular weight marker • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.• Verifying Western transfer efficiency.• Approximate sizing of proteins on SDS-PAGE and Western blots. Testing Data of Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) molecular weight marker TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP