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FN1 native protein :: Fibronectin Native Protein

Scan QR to view Datasheet Catalog #    MBS143461 FN1 native protein
Unit / Price
0.2 mg  /  $140 +1 FREE 8GB USB
1 mg  /  $205 +1 FREE 8GB USB
10 mg  /  $1,165 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fibronectin (FN1), Native Protein

 Also Known As   

Human Fibronectin

 Product Synonym Names    Fibronectin Human; Fibronectin Human; Fibronectin
 Product Gene Name   

FN1 native protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    Human Plasma
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 Purity/Purification    Greater than 95.0% as determined by SDS-PAGE.
 Form/Format    The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
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 Cell Culture Application    Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with a suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5ug/cm of growth surface.
 Solubility    Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours.
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 Preparation and Storage    Store the lyophilized fibronectin at 4 degree C. Upon reconstitution fibronectin should be stored at 4 degree C for 2 weeks and for future use below -18 degree C.Please prevent freeze-thaw cycles.
 Other Notes    Small volumes of FN1 native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for FN1 native protein

   Description: Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE. Fibronectin plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound Fibronectin receptors on cell surfaces. Molecular Weight 450kDa.
 Product Categories/Family for FN1 native protein    RECOMBINANT & NATURAL PROTEINS; Natural Proteins; Fibronectin
NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.
 NCBI GI #    74706043
 NCBI Accession #    O95608 [Other Products]
 Molecular Weight    13,341 Da [Similar Products]
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 NCBI Official Full Name    Fibronectin
 UniProt Protein Name    Fibronectin
 UniProt Synonym Protein Names   
Fibronectin
 Protein Family    Fibronectin
 UniProt Gene Name    FN1 [Similar Products]
 UniProt Entry Name    O95608_HUMAN
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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