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Enterohemorrhagic E Coli (EHEC) PCR Kit

Scan QR to view Datasheet Catalog #    MBS598057 Enterohemorrhagic E Coli (EHEC) pcr kit
Unit / Price
25 Tests/Kit (Instr. I, II Recommended)  /  $610 +1 FREE 8GB USB
25 Tests/Kit (Instr. III, IV Recommended)  /  $610 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Enterohemorrhagic E Coli (EHEC), PCR Kit

 Also Known As   

Enterohemorrhagic E. Coli (EHEC) Real Time PCR Kit

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Color Compensation Kit    Color Compensation Kit
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 Intended Uses    Enterohemorrhagic E. Coli (EHEC) real time PCR kit is used for the detection of Enterohemorrhagic E. Coli (EHEC) in stool or water samples by using real time PCR systems.
 Type of Instruments    Instrument I: LightCycler 1.0 (Internal control can't be used for this system)
Instrument II: LightCycler 2.0
Instrument III: PE5700, MJ-Opticon & other single color systems
Instrument IV: ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, iCycler iQ4, iCycler iQ5 & other multi-color systems
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 Preparation and Storage    All reagents should be stored at -20 degree C. Storage at +4 degree C is not recommended. Please refer to the pdf manual for more specific storage instructions.
 ISO Certification    Manufactured in an ISO 13485 Certified Laboratory.
 Other Notes    Small volumes of Enterohemorrhagic E Coli (EHEC) pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Enterohemorrhagic E Coli (EHEC) pcr kit

   Principle of the assay: The principle of the real-time detection is based on the fluorogenic 5'nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
 Product Categories/Family for Enterohemorrhagic E Coli (EHEC) pcr kit    Kit Series of Pathogen of Infectious Diarrhea
Sample Manual Insert of MBS598057
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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