View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Human hsa-mir-205 Real-Time RT-PCR PCR Kit Catalog # MBS825432 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name hsa-mir-205 Real-Time RT-PCR, PCR Kit Popular Item Full Product Name hsa-mir-205 Real-Time RT-PCR Detection and U6 Calibration Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Human Samples miRNA, Total RNA PCR Assay Type 2-Step qRT-PCR Detection Range Seven orders of magnitude Sensitivity 10 copies of a standard mimics and as little as 25 pg of total RNA Platform ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480 Components 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions. Dry Ice Shipment Extra charge fee may add to your shipping cost as dry ice is required to ship this product. Preparation and Storage Shipped at 4 degree C. Store at -20 degree C for 6 months. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of hsa-mir-205 Real-Time RT-PCR pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for hsa-mir-205 Real-Time RT-PCR pcr kit Detect mature miRNA hsa-mir-205 expression normalized by U6 snRNA. Applications Tested/Suitable for hsa-mir-205 Real-Time RT-PCR pcr kit Real-time RT-PCR Application Notes for hsa-mir-205 Real-Time RT-PCR pcr kit The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Amplication of hsa-mir-205 Real-Time RT-PCR pcr kit Synthesized standard amplifiction curve (10nM/100pM) Amplication #2 of hsa-mir-205 Real-Time RT-PCR pcr kit Amplifiction curve in total RNA from 293T/A549 cells Dissociation Curve of hsa-mir-205 Real-Time RT-PCR pcr kit Dissociation curve (Note: the minor peak on the left is the negative control) Electrophoresis of hsa-mir-205 Real-Time RT-PCR pcr kit Electrophoresis Image (20bp marker) TOP Sample Manual Insert of MBS825432. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP