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Mouse mmu-mir-21a-3p PCR Kit

Scan QR to view Datasheet Catalog #    MBS825782
Unit / Price
50 rxn  /  $395 +1 FREE 8GB USB
100 rxn  /  $570 +1 FREE 8GB USB
200 rxn  /  $825 +1 FREE 8GB USB
 
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 Product Name   

mmu-mir-21a-3p, PCR Kit

★Popular Item★
 Also Known As   

mmu-mir-21a-3p Real-Time RT-PCR Detection and U6 Calibration Kit

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for 6 months.
 Product Note    Our PCR Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of mmu-mir-21a-3p pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for mmu-mir-21a-3p pcr kit

   Principle of the Assay: miRNAs real-time Quantitation assay of miRNAs includes two steps, stem-loop RT and real-time PCR. Stem-loop RT primers bind to at the 3í portion of miRNA molecules and are reverse transcribed with reverse transcriptase. Then, the RT product is quantified using real-time PCR that includes miRNA-specific forward primer, reverse primer and SYBR Green dye.

Background/Introduction: The miRNAs RT-PCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples. miRNAs are small, single-stranded, ~19 - 23 nt RNA molecules encoded in the genomes of plants, animals, and viruses. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Unlike commonly used methods for detection of miRNAs, the miRNAs qPCR Quantitation Kit is more rapid and sensitive. This kit contains miRNA and U6 high specific RT and PCR primer set, with SYBR Green dye included. The stem-loop like miRNAs RT primer and the miRNAs high specific primer set ensure the RT and PCR reaction would not be interfered by the miRNAs precursors. The reaction template could be total RNA or cell lysates. The system enables highly sensitive detection from as few as 10 copies of a target miRNAs, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 ?g of total RNA. High specificity ensurenced by stemloop RT primer and high specific miRNAs primer set; even highly homogized miRNAs can be accurately dicriminated. Higher dynamic range and sensitivity of miRNAs quantification than conventional method (such as Northern blot & micro-array) with broad dynamic range of at least seven orders of magnitude, and in this ready to use formulation can quantify as few as 7 copies of a miRNA target in as little as 0.1 pg of total RNA. Less sample requirement Total RNA, cell lysate or purified samll RNA can work as the qPCR Quantitation Kitís template, even the genomic DNA contamination would not interfere in miRNAs quantification. miRNA synthetic standard can work as a standard curve when you need to know the absolute number of a miRNA in one cell or work as positive control when you need to know the relative expression ratio between miRNA and U6 snRNA or 5S rRNA.
 Applications Tested/Suitable for mmu-mir-21a-3p pcr kit   

Real-time RT-PCR

 Application Notes for mmu-mir-21a-3p pcr kit    The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
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 Amplication of mmu-mir-21a-3p pcr kit    Synthesized standard amplifiction curve (10nM/100pM)
mmu-mir-21a-3p pcr kit Amplication image
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 Amplication #2 of mmu-mir-21a-3p pcr kit    Amplifiction curve in total RNA from 293T/A549 cells
mmu-mir-21a-3p pcr kit Amplication #2 image
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 Dissociation Curve of mmu-mir-21a-3p pcr kit    Dissociation curve (Note: the minor peak on the left is the negative control)
mmu-mir-21a-3p pcr kit Dissociation Curve image
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 Electrophoresis of mmu-mir-21a-3p pcr kit    Electrophoresis Image (20bp marker)
mmu-mir-21a-3p pcr kit Electrophoresis image
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Sample Manual Insert of MBS825782. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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