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Hydroxysteroid Dehydrogenase, 11-beta Type II Peptide

Scan QR to view Datasheet Catalog #    MBS653968 Hydroxysteroid Dehydrogenase, 11-beta Type II peptide
Unit / Price
0.1 mg  /  $395 +1 FREE 8GB USB
 
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 Product Name   

Hydroxysteroid Dehydrogenase, 11-beta Type II, Peptide

★Popular Item★
 Also Known As   

Hydroxysteroid Dehydrogenase, 11-beta Type II, Human (11b-HSD2) (Control Peptide)

 Antibody/Peptide Pairs    Hydroxysteroid Dehydrogenase, 11-beta Type II peptide (MBS653968) is used for blocking the activity of Hydroxysteroid Dehydrogenase, 11-beta Type II antibody (MBS614847)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    Human synthetic peptide
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 Specificity    A 19aa synthetic peptide, mapping near the C-terminus of human 11beta-HSD2
 Purity/Purification    Highly purified
 Form/Format    Supplied as a liquid in PBS, pH 7.5.
 Concentration    1mg/ml (lot specific)
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 Species sequence homology    No significant sequence homology is seen with 11b-HSD1 or other proteins. No Significant homology with rat or mouse 11beta-HSD2
 Principle    It is not uncommon to see more than 1 band in Western Blot when probed with a given antibody or see more diffuse staining in immuno-Iocalization studies. The question arises which one of this band(s)/staining is specific. The antibody specificity is generally studied by competing with excess of antigen (peptide or protein) or immuno-neutralization with the antigens. In principle, a small volume of antibody (e.g. 1-5ul) is first reacted with excess peptide (5-50 fold over the antibody e.g., 1 ug antibody reacted with 5-50ug peptide; exact amounts determined by titration) to neutralize it. The neutralized antibody can no longer subsequently bind to another antigen (a band of interest) or staining pattern. So the band(s)/staining that is competed by the antigen/peptide is supposedly specific. If more than one band disappears by peptide/antigen competition then those bands have the antigenic determinants and could be considered either fragments of the large antigen or multimer.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of Hydroxysteroid Dehydrogenase, 11-beta Type II peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Hydroxysteroid Dehydrogenase, 11-beta Type II peptide

   Control Peptide for MBS614847 (antiserum) and MBS618093 (affinity purified) antibodies. 11-b-hydroxysteroid dehydrogenase (11b-HSD) is a microsomal short chain dehydrogenase/reductase (SDR) which catalyzes the inter-conversion of biologically active glucocorticoid (cortisol in human and corticosterone in rats and mice) and inactive glucocorticoid (cortisone and 11-dehydrocorticosterone). Two tissue specific isoforms (11b-HSD1 and 11b-HSD2) of 11b-HSD with two different functions regarding glucocorticoid availability, have been identified. The decreased 11b-hydroxy oxidation of cortisol results in Apparent Mineralocorticoid Excess (AME) disorder which is manifested by hypertension, hypokalemia, low plasma renin activity, and responsiveness to spironolactone. AME is principally a disorder of juveniles and children with this condition oxidize cortisol to cortisone poorly but carry out the reverse process unimpaired. AME arises from mutations in the 11b-HSD2 gene. The glucocorticoids can also be produced locally by 11b-HSD1 and increased visceral accumulation of glucocorticoids results in visceral obesity, insulin resistant diabetes, hyperlipidemia and hyperphagia. 11bHSD-2 (rat 400-aa, mouse 396-aa, human 405-aa) is a ~41kD glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11b-HSD2 are in the lumen and cytoplasm of ER, respectively. 11b-HSD2 irreversibly catalyzes the dehydrogenation of active 11b-hydroxycorticoids before they occupy mineralocorticoid receptors (MR) and thus confers aldosterone selectivity for inherently nonselective MR.
 Product Categories/Family for Hydroxysteroid Dehydrogenase, 11-beta Type II peptide    Molecular Biology; MB-Enzymes, Dehydrogenase
 Applications Tested/Suitable for Hydroxysteroid Dehydrogenase, 11-beta Type II peptide   

Elisa (EIA), Antibody Blocking

 Application Notes for Hydroxysteroid Dehydrogenase, 11-beta Type II peptide    Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. ELISA: 50-100ng control peptide/well. Antibody Blocking: 5-10ug per 1ug MBS618093 (affinity purified) or per 1ul MBS614847 (antiserum). Optimal dilutions to be determined by the researcher.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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