View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Unphosphorylated Dok1 (Ser-450) Peptide Catalog # MBS474064 Unit / Price 0.05 mg / $190 +1 FREE 8GB USB 5x0.05 mg / $690 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Unphosphorylated Dok1 (Ser-450) Peptide Full Product Name Unphosphorylated Dok1 (Ser-450) Peptide Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sequence Peptide Sequence: Unphosphor ylated Dok1 (Ser-450) synthetic peptide correspond s to amino acids surroundin g serine 450 in human Dok1. This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members. Specificity This peptide can be used as an unphosphorylated control peptide for Dok1 (Ser-450) antibody (DP2181) in ELISA, WB blocking, and ICC experiments. Form/Format Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide. Preparation and Storage Store at -20 degree C. Stable for 1 year. Other Notes Small volumes of Unphosphorylated Dok1 (Ser-450) Peptide peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Unphosphorylated Dok1 (Ser-450) Peptide peptide Doks are a family of adaptor proteins that recruit SH2-containing molecules involved in various cell signaling pathways. Six Dok proteins (Dok1 to Dok6) have been identified and each has an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras- GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IkB Kinase b phosphorylates several serine sites including Ser-450 in vitro, and TNF-alpha, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility. Application Notes for Unphosphorylated Dok1 (Ser-450) Peptide peptide Blocking: 1:1,000ELISA: 50 ng/well TOP Product References and Citations for Unphosphorylated Dok1 (Ser-450) Peptide peptide • Noguchi, T. et al. (1999) EMBOJ 18(7):1748.• Kashige, N. et al. (2000) Proc. Nat. Acad. Sci. 97(5):2093.• Lee, S. et al. (2004) Proc. Nat. Acad. Sci. 101(50):17416.• Woodring, P.J. (2004) J Cell Biol. 165(4):493. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP