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| Product Name
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phospho-EGFR (Ser-1142) Peptide (c-erbB proto-oncogene), Peptide
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| Also Known As
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phospho-EGFR (Ser-1142) Peptide
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sequence
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Peptide Sequence: Phospho-EG FR (Ser-1142) synthetic peptide correspond ing to amino acid residues surroundin g serine 1142 of human EGFR (ErbB-1). This human EGFR sequence has high homology with rat and mouse EGFR, and is not conserved in other ErbB family members.
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| 3D Structure
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ModBase 3D Structure for Q9P2Z9
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| Specificity
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The peptide is specifically recognized by EGFR (Ser-1142) antibody (EP1931) in ELISA, and has been shown to block the reactivity of EP1931 in Western blot and immunocytochemistry.
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| Form/Format
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Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
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| Preparation and Storage
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Store at -20 degree C. Stable for 1 year.
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| Other Notes
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Small volumes of c-erbB proto-oncogene peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.
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| Application Notes for c-erbB proto-oncogene peptide
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ELISA: 50 ng/well
Immunocytochemistry: 1:500
Western Blot: 1:1,000
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| NCBI/Uniprot data below describe general gene information for c-erbB proto-oncogene. It may not necessarily be applicable to this product. |
| NCBI GI #
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8815559
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| NCBI Accession #
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AAB19486.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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S51343.1
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[Other Products]
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| UniProt Primary Accession #
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Q9P2Z9
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[Other Products]
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| Molecular Weight
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1,020 Da
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[Similar Products]
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TOP
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| NCBI Official Full Name
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epidermal growth factor receptor
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| NCBI Official Symbol
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c-erbB proto-oncogene
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[Similar Products]
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| UniProt Protein Name
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Epidermal growth factor receptor
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| UniProt Gene Name
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c-erbB proto-oncogene
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[Similar Products]
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| UniProt Entry Name
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Q9P2Z9_HUMAN
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• Carpenter, G. (2000) Bioessays 22:697.
• Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with c-erbB proto-oncogene peptide | Pathways associated with c-erbB proto-oncogene peptide |
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TOP
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Peptide 
